The feasibility of non-viral gene transfer to the diaphragm in vivo
The feasibility of non-viral gene transfer to the diaphragm in vivo
复制标题
DOI：
10.1111/j.1440-169x.2009.01117.x
复制
发表时间：
2009-08-01
影响因子：
2.5
通讯作者：
Schmid, Ralph A.
中科院分区：
文献类型：
--
作者：
Beshay, Morris;Gazdhar, Amiq;Schmid, Ralph A.
使用电穿孔进行基因转移是研究发育生物学的重要方法，特别是了解肌肉细胞变性和凋亡的内部控制，这种变性和凋亡比通常由衰老引起的变性过程发生得更早和更快。此类实验研究可能有助于制定治疗患有遗传性原发性肌病（例如杜氏肌营养不良症（DMD））的患者的新策略。本研究旨在评估电穿孔介导的报告基因在体内转移至隔膜的可行性。这是首次报道使用电穿孔将裸露质粒 DNA 基因转移到体内膈肌中。我们的结果表明，使用电穿孔将裸质粒 DNA 体内基因转移到膈肌中是可行的。
Gene transfer using electroporation is an essential method for the study of developmental biology, especially to understand the internal control of degeneration and apoptosis of the muscle cells that occurs earlier and quicker than the usual degeneration process occurring by aging. Such experimental studies may have a role in developing new strategies for treating patients suffering from inherited primary myopathies such as Duchenne muscular dystrophy (DMD). The present study was designed to evaluate the feasibility of electroporation mediated transfer of reporter genes to the diaphragm in vivo. This is the first report of gene transfer of naked plasmid DNA into the diaphragm muscle in vivo using electroporation. Our results showed that in vivo gene transfer of naked plasmid DNA into the diaphragm muscle using electroporation is feasible.