Antagonistic activity of bacteria isolated from crops cultivated in a rotation system and a monoculture against Pythium debaryanum and Fusarium oxysporum
Antagonistic activity of bacteria isolated from crops cultivated in a rotation system and a monoculture against Pythium debaryanum and Fusarium oxysporum
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DOI：
10.1007/s12223-009-0062-1
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发表时间：
2009-09-01
影响因子：
2.6
通讯作者：
Stefaniak, O.
中科院分区：
文献类型：
--
作者：
Gorlach-Lira, K.;Stefaniak, O.
揭示了轮作和单作对对德巴氏腐霉和尖孢镰刀菌具有拮抗活性的细菌出现的影响。节杆菌属、荧光假单胞菌属放线菌是从冬油菜、甜菜和冬大麦的根际以及长期轮作试验（18年）的田块土壤中分离出来的。真菌拮抗分离株的出现及其抗菌水平表现出对地点、作物和轮作的特异性。真菌拮抗活性在放线菌和荧光假单胞菌属中很常见。在节杆菌属中较少见。荧光假单胞菌的抗菌作用。和节杆菌属。一般而言，对 P. debaryanum 的抵抗力强于 F. oxysporum。拮抗性假单胞菌属的百分比最高。大麦作物地块中存在针对 P. debaryanum 的拮抗剂，而冬油菜地块中针对 F. oxysporum 的拮抗剂出现频率较高。节杆菌属的抗菌活性最高。单一栽培的大麦和冬油菜菌株中均出现了针对这两种病原体的拮抗剂，而单一栽培的甜菜中这些细菌中没有尖孢镰刀菌拮抗剂。大多数放线菌分离株强烈抑制 P. debaryanum 和 F. oxysporum 的生长。拮抗放线菌比例及其抗菌水平在6年轮作制度中最高。
The effect of crop rotation and monocropping on the occurrence of bacteria with antagonistic activity toward Pythium debaryanum and Fusarium oxysporum was shown. Arthrobacter spp., fluorescent Pseudomonas spp. and actinomycetes were isolated from winter rape, sugar beet and winter barley rhizosphere and bulk soil from the plots of a long-term crop rotation experiment (18 years). The occurrence of mycoantagonistic isolates and their antibiosis level exhibited specificity for the site, crop and crop rotation. Mycoantagonistic activity was common among actinomycetes and fluorescent Pseudomonas spp. and less frequent among Arthrobacter spp. Antibiosis of fluorescent Pseudomonas spp. and Arthrobacter spp. was in general stronger against P. debaryanum than F. oxysporum. The highest percentage of antagonistic Pseudomonas spp. against P. debaryanum was in the plots of barley crop, while plots of winter rape showed higher frequency of antagonists against F. oxysporum. The highest antibiosis activity of Arthrobacter spp. against both pathogens occurred in isolates from barley and winter rape monoculture, and there were no F. oxysporum antagonists among these bacteria in sugar beet monoculture. Most of actinomycete isolates strongly inhibited growth of P. debaryanum and F. oxysporum. The percentage of mycoantagonistic actinomycetes and their antibiosis level were the highest in the 6-year crop rotation system.