Distinct expression and function of three ammonium transporter genes (OsAMT1;1-1;3) in rice
Distinct expression and function of three ammonium transporter genes (OsAMT1;1-1;3) in rice
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DOI：
10.1093/pcp/pcg083
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发表时间：
2003-07-01
影响因子：
4.9
通讯作者：
Yamaguchi, J
中科院分区：
文献类型：
--
作者：
Sonoda, Y;Ikeda, A;Yamaguchi, J
为了研究水稻根部铵吸收的调节，分离并检查了三个铵转运蛋白基因（OsAMT];1、1;2 和 1;3；Oryza sativa 铵转运蛋白）。 OsAMT1 属于 AMT1 家族，包含 11 个假定的跨膜结构域。 Southern blot分析和水稻基因组数据库筛选证实，通过OsAMT1;1-1;3，已经分离出完整的水稻AMT1家族。 OsAMT1 在酿酒酵母突变体 31019b 中的异源表达表明，所有三种 OsAMT1 均表现出铵转运活性。 Northern印迹分析显示这三个基因具有不同的表达模式； OsAMT1;1 在芽和根中的更多组成型表达，OsAMT1;2 的根特异性和铵诱导表达，以及 OsAMT1;3 的根特异性和氮抑制表达。原位 mRNA 检测显示 OsAMT1； 2表示在根尖的中央圆柱和细胞表面。该基因表达分析揭示了水稻中 AMT 的独特氮依赖性调节，与番茄或拟南芥中的不同。
To study the regulation of ammonium uptake into rice roots, three ammonium transporter genes (OsAMT];1, 1;2 and 1;3; Oryza sativa ammonium transporter) were isolated and examined. OsAMT1s belong to AMT1 family, containing 11 putative transmembrane-spanning domains. Southern blot analysis and screening of the rice genome database confirmed that with OsAMT1;1-1;3 the complete AMT1 family of rice had been isolated. Heterologous expression of OsAMT1s in the yeast Saccharomyces cerevisiae mutant 31019b showed that all three OsAMT1s exhibit ammonium transport activity. Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3. In situ mRNA detection revealed that OsAMT1; 2 is expressed in the central cylinder and cell surface of root tips. This gene expression analysis revealed a distinct nitrogen-dependent regulation for AMTs in rice, differing from that in tomato or Arabidopsis.