CTCF tethers an insulator to subnuclear sites, suggesting shared insulator mechanisms across species
CTCF tethers an insulator to subnuclear sites, suggesting shared insulator mechanisms across species
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DOI：
10.1016/s1097-2765(04)00029-2
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发表时间：
2004-01-30
期刊：
影响因子：
16
通讯作者：
Felsenfeld, G
中科院分区：
文献类型：
--
作者：
Yusufzai, TM;Tagami, H;Felsenfeld, G
绝缘子可以阻止一个基因的增强子激活附近另一个基因的启动子。几乎所有描述的脊椎动物绝缘子都需要结合调节蛋白CTCF来实现其活性。我们发现，CTCF copurifies与核仁蛋白nucleophosmin和两者都存在于绝缘子网站在体内。此外，外源绝缘子序列以CTCF依赖的方式被拴系到核仁。这些相互作用，完全不同的吉普赛绝缘子元件在果蝇，可能会产生类似的环结构，这表明一个共同的主题和模型的增强子阻断绝缘子的行动。
Insulators can block an enhancer of one gene from activating a promoter on another nearby gene. Almost all described vertebrate insulators require binding of the regulatory protein CTCF for their activity. We show that CTCF copurifies with the nucleolar protein nucleophosmin and both are present at insulator sites in vivo. Furthermore, exogenous insulator sequences are tethered to the nucleolus in a CTCF-dependent manner. These interactions, quite different from those of the gypsy insulator element in Drosophila, may generate similar loop structures, suggesting a common theme and model for enhancer-blocking insulator action.