A new continuous, monodimensional electrophoretic system for the separation and quantitation of individual glycosaminoglycans.
A new continuous, monodimensional electrophoretic system for the separation and quantitation of individual glycosaminoglycans.
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一种新的连续单维电泳系统，用于分离和定量单个糖胺聚糖。
DOI：
10.1016/0003-2697(81)90801-0
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发表时间：
1981
影响因子：
2.9
通讯作者：
Desnick,RJ
中科院分区：
文献类型：
--
作者：
Schuchman,EH;Desnick,RJ
建立了一种新的连续、一维醋酸纤维素电泳系统，用于分离哺乳动物的六种主要糖胺聚糖。硫酸角质素、硫酸乙酰肝素、硫酸皮肤素、肝素、4-硫酸软骨素和透明质酸可重复地分离，推测是基于它们在同一电泳溶液中与乙二胺四乙酸和锂阳离子上的氨基的差异结合。这种微量方法允许快速分离，直接比较，并在一个单一的电泳程序后，这些个别的糖胺聚糖的定量测定。
A new continuous, monodimensional cellulose acetate electrophoretic system was developed for the resolution of six major mammalian glycosaminoglycans. Keratan sulfate, heparan sulfate, dermatan sulfate, heparin, chondroitin 4-sulfate, and hyaluronic acid were reproductibly separated, presumably on the basis of their differential binding to the amino groups on ethylenediaminetetraacetic acid and lithium cations in the same electrophoretic solution. This micromethod permits the rapid separation, direct comparison, and quantitative determination of these individual glycosaminoglycans following a single electrophoretic procedure.