Effect of conduit repair on aberrant motor axon growth within the nerve graft in rats
Effect of conduit repair on aberrant motor axon growth within the nerve graft in rats
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DOI：
10.1002/micr.20394
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发表时间：
2007-01-01
期刊：
影响因子：
2.1
通讯作者：
Hosokawa, Ko
中科院分区：
文献类型：
--
作者：
Tomita, Koichi;Kubo, Tateki;Hosokawa, Ko
在周围神经损伤后，最大限度地减少轴突的错误方向已经成为获得良好功能结果的重要问题。一般来说，随着神经缺损长度的增加，其变得更具挑战性。由于以前的工作表明，管道修复留下一个短的差距，可以诱导一些目标特异性的神经再支配，我们预计，远端放置管道结合神经移植物将提高神经再支配的特异性，特别是在处理一个长的差距。为了测试这一点，在大鼠坐骨神经中产生14 mm长的间隙，并使用1）全神经移植物（WG）、2）束间神经移植物（FG）或3）全神经移植物与远端放置的硅胶管组合进行修复，留下5 mm间隙（TUBG）。在随访结束时，评估靶特异性神经再支配的程度（刺激坐骨神经及其胫束和腓总束诱发的复合肌肉动作电位的测量）和运动神经元投射的准确性（腓总运动池的顺序逆行标记）。两项评估均显示FG组和TUBG组具有相似的选择性，其显著高于WG组。与这些结果一致，通过步行轨迹分析评估的功能恢复显示FG组和TUBG组之间无显著差异，而FG组和TUBG组显著上级优于WG组。相反，再生轴突和湿肌肉重量的组织形态计量学评估显示三组之间无显著差异。总之，管道修复对减少运动轴突误导有一定作用，并且当与神经移植物联合用于治疗大缺陷时可能更有效。(C)2007 Wiley-Liss，Inc.
After peripheral nerve injury, minimizing axonal misdirection has been a matter of importance to obtain good functional outcomes. In general, it becomes more challenging as the nerve defect length is longer. As previous works suggested that a conduit repair leaving a short gap could induce some target-specific reinnervation, we expected that a distally placed conduit combined with nerve graft would enhance the specificity of reinnervation, especially in dealing with a long gap. To test this, a 14-mm-long gap was created in the rat sciatic nerves and repaired with either 1) whole nerve graft (WG), 2) interfascicular nerve grafts (FG), or 3) whole nerve graft combined with distally placed silicone tube leaving a 5-mm gap (TUBG). At the end of follow up, the extent of target specific reinnervation (measurement of the compound muscle action potentials evoked by stimulation of the sciatic nerve and its tibial and common peroneal fascicles) and the accuracy of motoneuronal projection (sequential retrograde labeling of the common peroneal motor pool) were assessed. Both assessments revealed that groups FG and TUBG had a similar selectivity, which was significantly higher than in group WG. Consistent with these results, the functional recovery as assessed by walking track analysis showed no significant difference between groups FG and TUBG, whereas those were significantly superior to group WG. In contrast, histomorphometric assessment of the regenerating axons and wet muscle weight showed no significant difference among the three groups. In conclusion, conduit repair would have some effects on reducing motor axonal misdirection, and it might be more effective when used in the management of a large defect in combination with nerve graft. (C) 2007 Wiley-Liss, Inc.