Host factors in nuclear plasmid maintenance in Saccharomyces cerevisiae.
Host factors in nuclear plasmid maintenance in Saccharomyces cerevisiae.
复制标题
酿酒酵母核质粒维持的宿主因素。
DOI：
10.1007/978-1-4684-5251-8_38
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Eisenberg,S
中科院分区：
文献类型：
--
作者：
Tye,BK;Sinha,P;Surosky,R;Gibson,S;Maine,G;Eisenberg,S
在酵母中，染色体上DNA复制的起始被认为发生在称为自主复制序列（ARS）的特定序列处。我们以前分离出一些突变体，是有缺陷的微型染色体的维护。对这些突变体的分析表明，尽管ARSs的一级序列彼此差异很大，但它们似乎共享一个共同的酶复合物来启动DNA复制。然而，这种起始酶复合物可能以不同的亲和力与不同的ARS结合。我们发现了一种ARS结合蛋白，这种蛋白以不同的效率与不同的ARS结合，从而证实了这一观点。
In yeast, the initiation of DNA replication on chromosomes is believed to occur at specific sequences known as autonomously replicating sequences (ARSs). We previously isolated a number of mutants that are defective in the maintenance of minichromosomes. Analysis of these mutants suggests that althoughARSs differ greatly from one another in their primary sequences, they appear to share a common enzyme complex for the initiation of DNA replication. However, this initiation enzyme complex probably binds with differential affinity to differentARSs. This idea is corroborated by our identification of anARS-binding protein that binds to differentARSs with different efficiencies.