Role of the renal nerves in the control of renin synthesis during different sodium intakes in the rat
Role of the renal nerves in the control of renin synthesis during different sodium intakes in the rat
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DOI：
10.1097/00004872-200107000-00012
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发表时间：
2001-07-01
影响因子：
4.9
通讯作者：
Zanchetti, A
中科院分区：
文献类型：
--
作者：
Golin, R;Pieruzzi, F;Zanchetti, A
目的 探讨肾神经在不同钠平衡状态下正常血压大鼠肾素合成的调节作用。方法48只雄性Sprague-Dawley大鼠分为6个实验组，结合3种不同NaCl含量（正常0.4%、低0.04%或高4.0%）的饮食，并进行手术、双侧肾脏去神经术或假手术。饮食治疗7 d后，处死所有大鼠，测定血浆肾素活性（PRA），半定量聚合酶链反应测定肾皮质肾素信使RNA（mRNA）水平，结果与标准饮食组相比，低钠饮食组PRA较高，高钠饮食组PRA较低。去肾神经可以降低正常钠组和低钠组的 PRA，但不会改变高钠组的 PRA 值。与标准饮食组相比，低钠饮食组的肾素基因表达显着升高，高钠饮食组的肾素基因表达显着降低。去肾神经可以显着降低接受低钠饮食的大鼠的肾素mRNA水平，但在正常或高钠组中没有产生任何显着变化。 结论 大鼠缺钠期间肾素基因表达的激活依赖于肾神经的存在，而钠负荷期间肾素基因表达的抑制似乎仅归因于致密斑机制。 (C) 2001 年利平科特威廉姆斯和威尔金斯。
Objective The aim of this study was to evaluate the role of the renal nerves in the regulation of renin synthesis in normotensive rats at different sodium balance.Methods Forty-eight male Sprague-Dawley rats were divided in six experimental groups, combining three diets at different NaCl content (normal 0.4%, low 0.04% or high 4.0%), and the surgical, bilateral renal denervation or the sham procedure. After 7 days of dietary treatment, all rats were sacrificed and plasma renin activity (PRA) was measured, Renin messenger RNA (mRNA) levels in the renal cortex were determined by semiquantitative polymerase chain reaction,Results PRA was higher in animals fed the low sodium diet compared with those at standard diet, while it was lower in animals fed the high sodium diet. Renal denervation decreased PRA in normal and low sodium groups, while it did not alter the PRA values in the high sodium group. Renin gene expression significantly increased in rats fed with the low sodium diet compared with the standard diet group, and significantly decreased in rats fed the high sodium diet. Renal denervation significantly reduced renin mRNA levels in rats receiving the low sodium diet, but did not produce any significant change in normal or high-sodium groups.Conclusion The activation of renin gene expression during sodium depletion in rats is dependent on the presence of the renal nerves, while the suppression of renin gene expression during a sodium load seems to be due to the macula densa mechanism alone. (C) 2001 Lippincott Williams & Wilkins.