Expression of matrix metalloproteinase-9 mRNA and protein during deciduous tooth resorption in bovine odontoclasts
Expression of matrix metalloproteinase-9 mRNA and protein during deciduous tooth resorption in bovine odontoclasts
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DOI：
10.1016/s8756-3282(02)00856-6
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发表时间：
2002-10-01
期刊：
影响因子：
4.1
通讯作者：
Shimokawa, H
中科院分区：
文献类型：
--
作者：
Linsuwanont, B;Takagi, Y;Shimokawa, H
基质金属蛋白酶-9（MMP-9 或明胶酶 B）是一种细胞外蛋白酶，在破骨细胞中高表达，并被认为在破骨细胞的再吸收活性中发挥重要作用。尽管据报道 MMP-9 在骨吸收中发挥作用，但该酶在乳牙吸收过程中的关联尚未明确。本研究的目的是加深我们对 MMP-9 在乳牙吸收过程中作用的了解。对从位于乳牙根部及其恒牙继承体之间的牛根吸收组织中提取的总 RNA 进行逆转录聚合酶链反应 (RT-PCR) 和 Northern 印迹分析，揭示了组织中 MMP-9 的 hnRNA 表达。这些结果表明MMP-9可能参与乳牙吸收过程。此外，还进行了原位杂交和免疫组织化学鉴定牛根吸收组织中产生MMP-9的细胞。 MMP-9 mRNA 在沿组织表面排列的破牙细胞中高度表达。免疫组织化学证实了 MMP-9 在破牙细胞中的主要定位。目前的数据表明，乳牙牙根吸收中的破牙细胞表达MMP-9，其可能参与乳牙牙根吸收过程中的蛋白水解。
Matrix metalloproteinase-9 (MMP-9, or gelatinase B) is an extracellular proteinase that is highly expressed in osteoclasts and has been postulated to play an important role in their resorptive activity. Although MMP-9 has been reported to play a role in bone resorption, the association of this enzyme during deciduous tooth resorption has not yet been clarified. The purpose of the present study was to increase our understanding of the role of MMP-9 during deciduous tooth resorption. Reverse transcription-polymerase chain reaction (RT-PCR) and northern blot analysis of total RNAs extracted from bovine root-resorbing tissues, which lie between the root of a deciduous tooth and its permanent successor, revealed the expression of hnRNA for MMP-9 in the tissue. These results indicate that MMP-9 may be involved in the process of deciduous tooth resorption. In addition, in situ hybridization and immunohistochemistry were also performed to identify the cells that produced MMP-9 in bovine root-resorbing tissue. MMP-9 mRNA was highly expressed in odontoclasts that were aligned along the surface of the tissue. Immunohistochemistry confirmed the predominant localization of MMP-9 in odontoclasts. The present data demonstrate that odontoclasts in deciduous root resorption express MMP-9, which may participate in proteolysis during root resorption of deciduous tooth.