Neurofilament proteins are preferentially expressed in descending output neurons of the cat the superior colliculus: A study using SMI-32
Neurofilament proteins are preferentially expressed in descending output neurons of the cat the superior colliculus: A study using SMI-32
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DOI：
10.1016/j.neuroscience.2005.11.045
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发表时间：
2006-01-01
期刊：
影响因子：
3.3
通讯作者：
McHaffie, JG
中科院分区：
文献类型：
--
作者：
Fuentes-Santamaria, V;Stein, BE;McHaffie, JG
生理学研究表明，猫上丘的多感觉（即中间和深层）层的输出神经元接收来自神经轴的广泛区域的汇聚信息，整合这些信息，然后将产品传递给脑干参与控制头部和眼睛运动的区域。然而，由于许多末梢接触远端树突，很难用通常用于可视化上丘输出神经元的神经化学标记来标记，因此对这些汇聚传入事件的神经解剖学理解受到阻碍。在这里，我们发现SMI-32抗体针对高分子量神经丝蛋白的非磷酸化表位，是这些上丘输出神经元的有效标记。它也可以标记他们的远端树突。SMI-32处理的上丘切片显示，在深椎板内有许多不同形态的标记神经元。相比之下，在浅层中很少观察到标记神经元。深上丘外侧大体的神经元被标记得特别好，它们的许多二级和三级树突都清晰可见。将荧光生物素化葡聚糖胺注射到脑桥网状结构中显示，大约80%的SMI-32免疫染色神经元也含有逆行运输的生物素化葡聚糖胺，这表明SMI-32是下行输出神经元中表达的常见细胞骨架成分。已知上丘输出神经元也表达钙结合蛋白小白蛋白，许多SMI-32免疫染色的神经元也被证明是小白蛋白免疫染色。这些研究表明，SMI-32可以作为一种有用的免疫组织化学标记物，用于详细描述上丘输出神经元的体细胞和树突形态，并有助于评估它们的输入/输出关系。(c) 2005 IBRO。Elsevier Ltd.出版。版权所有。
Physiological studies indicate that the output neurons in the multisensory (i.e. intermediate and deep) laminae of the cat superior colliculus receive converging information from widespread regions of the neuraxis, integrate this information, and then relay the product to regions of the brainstem involved in the control of head and eye movements. Yet, an understanding of the neuroanatomy of these converging afferents has been hampered because many terminals contact distal dendrites that are difficult to label with the neurochemical markers generally used to visualize superior colliculus output neurons. Here we show that the SMI-32 antibody, directed at the non-phosphorylated epitopes of high molecular weight neurofilament proteins, is an effective marker for these superior colliculus output neurons. It is also one that can label their distal dendrites. Superior colliculus sections processed for SMI-32 revealed numerous labeled neurons with varying morphologies within the deep laminae. In contrast, few labeled neurons were observed in the superficial laminae. Neurons with large somata in the lateral aspects of the deep superior colliculus were particularly well labeled, and many of their secondary and tertiary dendrites were clearly visible. Injections of the fluorescent biotinylated dextran amine into the Pontine reticular formation revealed that approximately 80% of the SMI-32 immuno-stained neurons also contained retrogradely transported biotinylated dextran amine, indicating that SMI-32 is a common cytoskeletal component expressed in descending output neurons. Superior colliculus output neurons also are known to express the calcium-binding protein parvalbumin, and many SMI-32 immuno-stained neurons also proved to be parvalbumin immunostained. These studies suggest that SMI-32 can serve as a useful immunohistochemical marker for detailing the somatic and dendritic morphology of superior colliculus output neurons and for facilitating evaluations of their input/output relationships. (c) 2005 IBRO. Published by Elsevier Ltd. All rights reserved.