One-Step Thermophoretic AND Gate Operation on Extracellular Vesicles Improves Diagnosis of Prostate Cancer
One-Step Thermophoretic AND Gate Operation on Extracellular Vesicles Improves Diagnosis of Prostate Cancer
复制标题
细胞外囊泡一步热泳和门操作改善前列腺癌的诊断
DOI：
10.1002/anie.202207037
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发表时间：
2022-07-11
影响因子：
16.6
通讯作者：
Sun, Jiashu
中科院分区：
文献类型：
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作者：
Deng, Jinqi;Zhao, Shuai;Sun, Jiashu
循环细胞外囊泡（EV）已经成为癌症生物标志物的有价值的来源。然而，EV的高度异质性和临床样本的复杂性对肿瘤源性EV的灵敏鉴定提出了挑战。在这里，我们介绍了一个一步热泳与门操作（探戈）分析，集成了聚乙二醇（PEG）增强的热泳积累的EV和同时与门操作的EV膜上的双适体识别。通过使用Tango测定以均匀、无分离的形式直接从血清中检测EpCAM和PSMA共存的肿瘤源EV，我们可以在诊断灰色区中区分前列腺癌（PCa）患者与良性前列腺增生（BPH）患者，15分钟内准确率为91%。我们的方法在单个测定中简化了EV富集和对EV的与门操作，为癌症的精确和非侵入性诊断提供了快速、直接和有力的方法。
Circulating extracellular vesicles (EVs) have emerged as a valuable source of cancer biomarkers. However, the high degree of EV heterogeneity and the complexity of clinical samples pose a challenge in the sensitive identification of tumor-derived EVs. Here we introduce a one-step thermophoretic AND gate operation (Tango) assay that integrates polyethylene glycol (PEG)-enhanced thermophoretic accumulation of EVs and simultaneous AND gate operation on EV membranes by dual-aptamers recognition. By using the Tango assay to detect tumor-derived EVs with co-presence of EpCAM and PSMA directly from serum in a homogeneous, separation-free format, we can discriminate prostate cancer (PCa) patients from benign prostatic hyperplasia (BPH) patients in the diagnostic gray zone with an accuracy of 91 % in 15 min. Our approach streamlines EV enrichment and AND gate operation on EVs in a single assay, providing a rapid, straightforward, and powerful method for precise and non-invasive diagnosis of cancer.