Accumulation of the early histone messenger RNAs during the development of Strongylocentrotus purpuratus.
Accumulation of the early histone messenger RNAs during the development of Strongylocentrotus purpuratus.
复制标题
紫圆甲藻发育过程中早期组蛋白信使 RNA 的积累。
DOI：
10.1016/0012-1606(82)90360-8
复制
发表时间：
1982
影响因子：
2.7
通讯作者：
F. Wilt
中科院分区：
文献类型：
--
作者：
Robert E. Maxson;F. Wilt
测定早期组蛋白的mRNA量的发育概况。用~ 3 H核苷标记海胆胚胎，提取RNA，聚丙烯酰胺凝胶电泳分离RNA。测定分离mRNA条带中的放射性，并通过了解前体池比活度将其转换为摩尔量。在16- 200细胞期之间，每个核心组蛋白mRNA积累7-10 × 106个分子，H1 mRNA积累2.5 × 105个分子。在随后的几个小时的切割过程中，积累的mRNA消失，半衰期为1.5-2小时。有人认为，mRNA的半衰期可能是在切割过程中调节。
The developmental profile of amounts of the mRNAs for the early histones was determined. Sea urchin embryos (Strongylocentrotus purpuratus) were labeled with3H nucleoside, and the RNA was extracted and fractionated by electrophoresis on polyacrylamide gels. Radioactivity in the resolved mRNA bands was determined and converted to molar quantities by knowledge of the precursor pool-specific activity. Between the 16- and the 200-cell stages 7–10 × 106molecules of each core histone mRNA, and 2.5 × 105molecules of H1 mRNA accumulate. During the subsequent few hours of cleavage the accumulated mRNAs disappear with a half-life of 1.5–2 hr. It is argued that mRNA half-life may be regulated during cleavage.