Electron transport to nitrogenase in Rhodospirillum rubrum: The role of NAD(P)H as electron donor and the effect of fluoroacetate on nitrogenase activity
Electron transport to nitrogenase in Rhodospirillum rubrum: The role of NAD(P)H as electron donor and the effect of fluoroacetate on nitrogenase activity
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DOI：
10.1111/j.1574-6968.1997.tb10379.x
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发表时间：
1997-05-15
影响因子：
2.1
通讯作者：
Nordlund, S
中科院分区：
文献类型：
--
作者：
Brostedt, E;Lindblad, A;Nordlund, S
本文研究了红杜鹃固氮酶中TCA循环反应在还原剂产生中的作用。添加氟乙酸抑制固氮酶活性时，丙酮酸或内源性来源被用作电子供体几乎完全，而苹果酸，琥珀酸或富马酸时，抑制是不完全的。添加NAD（P）H到细胞中支持固氮酶活性，无论是否预先添加氟乙酸。我们认为，TCA循环在固氮中的作用在R。红色是产生还原的吡啶核苷酸，其被电子传递途径的组分氧化成固氮酶。
The role of the reactions of the TCA cycle in the generation of reductant for nitrogenase in Rhodospirillum rubrum has been investigated. Addition of fluoroacetate inhibited nitrogenase activity almost completely when pyruvate or endogenous sources were used as electron donors, whereas the inhibition was incomplete when malate, succinate or fumarate were used. Addition of NAD(P)H to cells supported nitrogenase activity, both with and without prior addition of fluoroacetate. We suggest that the role of the TCA cycle in nitrogen fixation in R. rubrum is to generate reduced pyridine nucleotides which are oxidized by the components of the electron transport pathway to nitrogenase.