Prolonged treatment of fair-skinned mice with topical forskolin causes persistent tanning and UV protection.
Prolonged treatment of fair-skinned mice with topical forskolin causes persistent tanning and UV protection.
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长期使用局部毛喉素治疗白皮肤小鼠会导致持续晒黑和紫外线防护。
DOI：
10.1111/j.1755-148x.2008.00536.x
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发表时间：
2009
影响因子：
4.3
通讯作者：
D'Orazio,JohnA
中科院分区：
文献类型：
--
作者：
Spry,MalindaL;Vanover,JillianC;Scott,Timothy;Abona-Ama,Osama;Wakamatsu,Kazumasa;Ito,Shosuke;D'Orazio,JohnA
我们以前报道过，局部应用毛喉素到皮肤白皙的MC1R缺陷小鼠的皮肤与表皮黑素细胞导致真黑素在表皮中的积累，并高度保护免受紫外线介导的皮肤损伤。在这份报告中，我们描述了长期局部毛喉素治疗在这种动物模型的长期影响。毛喉素诱导的真黑素产生持续3个月的每日应用，毛喉素诱导的真黑素仍然对紫外线损伤的保护作用，通过最小吸收剂量（MED）进行评估。 在暴露于长期毛喉素治疗的动物的皮肤或整体健康状况中未观察到明显的毒性变化。在局部毛喉素应用的整个过程中保持体重。局部应用毛喉素与表皮中黑素细胞数量的增加和表皮增厚有关，至少部分是由于有核角质形成细胞的积累。总之，这些数据表明，在该动物模型中，短期局部定期应用毛喉素可促进真黑素诱导，并且随着时间的推移，局部毛喉素治疗与持续黑化、表皮细胞蓄积和皮肤增厚相关。
We previously reported that topical application of forskolin to the skin of fair‐skinned MC1R‐defective mice with epidermal melanocytes resulted in accumulation of eumelanin in the epidermis and was highly protective against UV‐mediated cutaneous injury. In this report, we describe the long‐term effects of chronic topical forskolin treatment in this animal model. Forskolin‐induced eumelanin production persisted through 3 months of daily applications, and forskolin‐induced eumelanin remained protective against UV damage as assessed by minimal erythematous dose (MED). No obvious toxic changes were noted in the skin or overall health of animals exposed to prolonged forskolin therapy. Body weights were maintained throughout the course of topical forskolin application. Topical application of forskolin was associated with an increase in the number of melanocytes in the epidermis and thickening of the epidermis due, at least in part, to an accumulation of nucleated keratinocytes. Together, these data suggest in this animal model, short‐term topical regular application of forskolin promotes eumelanin induction and that over time, topical forskolin treatment is associated with persistent melanization, epidermal cell accumulation, and skin thickening.