Induced Pluripotent Stem Cells for Regenerative Medicine: Quality Control Based on Evaluation of Lipid Composition.
Induced Pluripotent Stem Cells for Regenerative Medicine: Quality Control Based on Evaluation of Lipid Composition.
复制标题
用于再生医学的诱导多能干细胞：基于脂质成分评估的质量控制。
DOI：
10.1007/5584_2019_394
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Shimizu Y.
中科院分区：
文献类型：
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作者：
Nakamura Y;Shimizu Y.
诱导多能干细胞（iPSCs）可以分化为多种功能细胞，其临床应用正在进行中，一些临床试验已经完成或正在进行中。另一方面，致癌风险是一个问题，细胞重编程的机制仍然未知。当多能干细胞和分化细胞用于医疗应用时，质量控制也很重要。磷脂不仅是脂质双层膜的结构成分，而且具有多种生理功能，因此我们讨论了通过评价磷脂来进行iPSCs质量控制的可能性。最近，脂质分析的方法变得更加广泛和容易执行。本文综述了诱导多能干细胞在再生医学中的作用，并探讨了利用磷脂进行诱导多能干细胞和分化细胞质量控制的可能性。
Clinical application of induced pluripotent stem cells (iPSCs), which can be differentiated into a wide variety of functional cells, is underway and some clinical trials have already been performed or are ongoing. On the other hand, the risk of carcinogenesis is an issue and the mechanism of cellular reprograming remains unknown. When iPSCs and differentiated cells are used for medical applications, quality control is also important. Here we discuss the possibility of performing quality control of iPSCs by evaluation of phospholipids, which are not just structural components of lipid bilayer membranes, but also have multiple physiological functions. Recently, methods for analysis of lipids have become more widely available and easier to perform. This article reviews the role of iPSCs in regenerative medicine and examines the possibility of using phospholipids for quality control of iPSCs and differentiated cells.