Development of biodegradable scaffolds based on magnetically guided assembly of magnetic sugar particles
Development of biodegradable scaffolds based on magnetically guided assembly of magnetic sugar particles
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DOI：
10.1016/j.jbiotec.2012.02.002
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发表时间：
2012-05-31
影响因子：
4.1
通讯作者：
Kwon, Guiryong
中科院分区：
文献类型：
--
作者：
Hu, Chengzhi;Uchida, Tomoyuki;Kwon, Guiryong
可生物降解支架具有可控制的孔隙布局和孔隙度，在组织工程中对细胞渗透、组织长入、血管化和营养输送具有重要意义。孔隙浸出是控制支架孔隙大小、孔隙结构和孔隙率的常用方法。在本文中，我们着重于使用/发展两种磁引导多孔材料组装方法，利用磁糖颗粒（MSPs）来制造支架。首先，利用图案化装置根据设计的模板对msp进行对齐。然后，将聚乙烯醇、聚乙烯醇和钕铁硼粉末混合制成磁性薄膜，用于引导msp。聚l-丙交酯-共ε -己内酯（PLCL）浇铸去除糖模板后，得到了具有球形孔的支架。利用光镜和电子显微镜对支架的表面和内部结构进行了评价，显示了它们的孔隙互连、孔壁形态和孔隙率。单层支架尺寸为8mm宽，10mm长，孔径范围为105- 150mu m， 250- 300mu m和425- 500mu m。(C) 2012 Elsevier B.V.版权所有。
Biodegradable scaffolds with controlled pore layout and porosity have great significance in tissue engineering for cell penetration, tissue ingrowth, vascularization, and nutrient delivery. Porogen leaching has been commonly used to control pore size, pore structure and porosity in the scaffold. In this paper we focus on the use/development of two magnetically guided porogen assembly methods using magnetic sugar particles (MSPs) for scaffold fabrication. First, a patterning device is utilized to align MSPs following designed templates. Then a magnetic sheet film is fabricated by mixing poly(vinyl alcohol, PVA) and NdFeB powder for steering the MSPs. After poly(l-lactide-co-epsilon-caprolactone) (PLCL) casting and removal of the sugar template, a scaffold with spherical pores is obtained. The surface and the inner structure of the scaffolds are evaluated using light and electron micrographs showing their interconnection of pores, pore wall morphology and porosity. Single layer scaffolds with the size of 8 mm in width and 10 mm in length were constructed with controllable pore diameters in the ranges of 105-150 mu m, 250-300 mu m and 425-500 mu m. (C) 2012 Elsevier B.V. All rights reserved.