Repeated immunosensing by a dithiobis(succinimidyl propionate)-modified SAW device
Repeated immunosensing by a dithiobis(succinimidyl propionate)-modified SAW device
复制标题
通过二硫双（琥珀酰亚胺基丙酸酯）修饰的 SAW 装置进行重复免疫传感
DOI：
10.1016/j.talanta.2019.05.080
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发表时间：
2019
期刊：
影响因子：
6.1
通讯作者：
Mitsubayashi Kohji
中科院分区：
文献类型：
--
作者：
Toma Koji;Oishi Koki;Yoshimura Naoyuki;Arakawa Takahiro;Yatsuda Hiromi;Mitsubayashi Kohji
探讨了二硫代双（琥珀酰亚胺基丙酸酯）（DSP）用于声表面波免疫传感器重复免疫分析的可能性。在传感器中，DSP被用来修改一个金涂层的SAW器件的石英敏感区，通过形成一个自组装单分子层的黄金表面。在使用小鼠（mIgG）和抗小鼠（a-mIgG）抗体的模型夹心测定中，第一抗体mIgG首先与DSP的N-羟基琥珀酰亚胺酯基团反应，并固定在SAW器件上以制备SAW免疫传感器。对mIgG吸附时间的优化表明，mIgG的吸附和固定化程度在30 min时均达到饱和，但固定化程度更依赖于吸附时间。通过对DSP修饰的SAW免疫传感器的表征，获得了高选择性，除了a-mIgG之外，没有从各种第二抗体观察到传感器输出，沿着具有比酶联免疫吸附测定短8倍的测量时间（15 min）。此外，α-mIgG的10次重复测量证明了传感器输出的高再现性（变异系数为7.0%）。这些验证了DSP在SAW免疫传感器中的实用性，用于快速和重复测量抗原。
The possibility of dithiobis(succinimidyl propionate) (DSP) for repeated immunoassay with a surface acoustic wave (SAW) immunosensor was explored. In the sensor, DSP was used to modify a gold-coated quartz sensing area of a SAW device by forming a self-assembled monolayer on the gold surface. In a model sandwich assay using mouse (mIgG) and anti-mouse (a-mIgG) antibodies, the primary antibody, mIgG, firstly reacted with N-hydroxysuccinimide ester groups of DSP and was immobilized on the SAW device to fabricate the SAW immunosensor. Optimization of adsorption time of mIgG revealed that both degrees of adsorption and immobilization of mIgG reached a saturation at 30 min although the immobilization was more dependent on the adsorption time. Through characterization of the DSP-modified SAW immunosensor, a high selectivity, with which no sensor output was observed from various kinds of secondary antibodies except for a-mIgG, along with 8-fold shorter measurement time (15 min) than that of enzyme-linked immunosorbent assay were obtained. Furthermore, 10 repeated measurement of a-mIgG demonstrated a high reproducibility of the sensor output (coefficient of variation of 7.0%). These validate the utility of DSP in the SAW immunosensor for rapid and repeated measurement of antigens.