EFFECTS OF MAJOR AND MINOR TRAUMA ON LYMPHOCYTE KINETICS IN MICE
EFFECTS OF MAJOR AND MINOR TRAUMA ON LYMPHOCYTE KINETICS IN MICE
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DOI：
10.1038/icb.1979.49
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发表时间：
1979-01-01
期刊：
影响因子：
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通讯作者：
DONALD, KJ
中科院分区：
文献类型：
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作者：
BOLTON, PM;KIROV, SM;DONALD, KJ
观察了重伤和轻伤对C57BL小鼠外周血白细胞数的影响。不仅是严重的创伤(肾切除)，而且轻微的创伤和压力(如注射、出血)导致血液中循环的淋巴细胞数量显著下降(50%-70%)。下降是缓慢的，最大降幅出现在手术或搬运后2小时。严重创伤导致B[骨髓源性]和T[胸腺源性]淋巴细胞下降。轻微创伤仅导致B淋巴细胞下降。循环多形体数量增加3-4倍也伴随着重大创伤，但在轻微创伤后没有观察到增加。血象一般在轻伤和重伤后24小时内恢复正常。在康复后重复创伤刺激会导致新的创伤反应。双侧肾上腺切除使淋巴细胞对大创伤和小创伤的反应消失，对大创伤的多形性反应减少50%以上，表明应激激素在这些变化中起作用。用51C标记的淋巴细胞转移到创伤和肾上腺切除动物中的研究表明，淋巴细胞进入血液的减少(而不是从血液进入组织或细胞死亡的增加)是创伤后淋巴细胞减少的最可能机制。
The effects of major and minor trauma on the circulating white blood cell populations of C57BL mice were followed. Not only major trauma (nephrectomy) but minor injury and stress (e.g., injection, bleeding) triggered a highly significant fall (50-70%) in number of lymphocytes circulating in the blood. The fall was gradual, with the maximal drop 2 h after the operation or handling procedure. Major trauma resulted in a fall in B [bone marrow-derived] and T [thymus-derived] lymphocytes. Minor trauma produced a fall in B lymphocytes only. A 3-4 fold increase in circulating polymorph numbers also accompanied major trauma, but no increase was observed after minor trauma. The blood picture returned to normal generally within 24 h of both minor and major trauma. Repetition of the trauma stimulus after recovery led to a renewed trauma response. Bilateral adrenalectomy abolished the lymphocyte response to major and minor trauma and decreased the polymorph response to major trauma by more than 50%, indicating that stress hormones played a role in these changes. Studies with 51C-labeled lymphocytes, transferred into traumatized and adrenalectomized animals, suggested that decreased entry of lymphocytes into the blood (rather than increased exit from the blood into the tissues, or cell death) was the most likely mechanism of the lymphopenia following trauma.