Gene expression profiles in peripheral blood mononuclear cells reflect the pathophysiology of type 2 diabetes
Gene expression profiles in peripheral blood mononuclear cells reflect the pathophysiology of type 2 diabetes
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DOI：
10.1016/j.bbrc.2007.07.006
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发表时间：
2007-09-21
影响因子：
3.1
通讯作者：
Kaneko, Shuichi
中科院分区：
文献类型：
--
作者：
Takamura, Toshinari;Honda, Masao;Kaneko, Shuichi
我们假设，系统循环外周血单核细胞（PBMC）反映了2型糖尿病的病理生理。从18名2型糖尿病患者和16名非糖尿病受试者中获得PBMC。通过使用DNA芯片分析PBMC中基因的表达，然后对生物学类别的特定基因集进行统计分析。唯一的基因集协调上调的存在糖尿病和下调血糖控制包括48个基因参与c-Jun N-末端激酶（JNK）途径。与此相反，唯一的基因集协调下调的存在糖尿病，但不改变血糖控制包括92个基因参与线粒体氧化磷酸化（OXPHOS）途径。我们的研究结果表明，参与JNK和OXPHOS通路的PBMC的基因可能是高血糖诱导的氧化应激和2型糖尿病的发病率，分别替代转录标志物。(c)2007爱思唯尔公司All rights reserved.
We hypothesized that systemically circulating peripheral blood mononuclear cells (PBMCs) reflect the pathophysiology of type 2 diabetes. PBMCs were obtained from 18 patients with type 2 diabetes and 16 non-diabetic subjects. The expression of genes in the PBMCs was analyzed by using a DNA chip followed by statistical analysis for specific gene sets for biological categories. The only gene set coordinately up-regulated by the existence of diabetes and down-regulated by glycemic control consisted of 48 genes involved in the c-Jun N-terminal kinase (JNK) pathway. In contrast, the only gene set coordinately down-regulated by the existence of diabetes, but not altered by glycemic control consisted of 92 genes involved in the mitochondrial oxidative phosphorylation (OXPHOS) pathway. Our findings suggest that genes involved in the JNK and OXPHOS pathways of PBMCs may be surrogate transcriptional markers for hyperglycemia-induced oxidative stress and morbidity of type 2 diabetes, respectively. (c) 2007 Elsevier Inc. All rights reserved.