Quorum quenching and antimicrobial activity of goldenseal (Hydrastis canadensis) against methicillin-resistant Staphylococcus aureus (MRSA).
Quorum quenching and antimicrobial activity of goldenseal (Hydrastis canadensis) against methicillin-resistant Staphylococcus aureus (MRSA).
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DOI：
10.1055/s-0032-1315042
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发表时间：
2012-09
期刊：
影响因子：
2.7
通讯作者：
Horswill AR
中科院分区：
文献类型：
--
作者：
Cech NB;Junio HA;Ackermann LW;Kavanaugh JS;Horswill AR
广受欢迎的中草药加拿大水仙(Hydrastis canamsis L.)传统上是用来治疗皮肤感染的。在这项研究中，我们展示了加拿大隐翅虫提取物在体外对耐甲氧西林金黄色葡萄球菌(MRSA)的活性。加拿大黄连叶提取物显示出比生物碱黄连素更强的抗菌活性(MIC值分别为75微克/毫升和150微克/毫升)。LC-MS检测到提取物中的生物碱和外排抑制黄酮类化合物，后者可能解释了提取物与黄连素单独使用相比具有增强药效的原因。我们还展示了抗毒力活性的证据，作为加拿大H.canensis对金黄色葡萄球菌作用的第二种机制。加拿大毛细管藻叶提取物(但不包括分离的生物碱黄连素、海藻氨酸和斑腿草碱)对几个临床相关的MRSA分离株(USA300菌株)显示出法定人数猝灭活性。我们的数据表明，这是由于通过AGCA双组分系统的信号转导减弱而发生的。与此一致的是，该提取物抑制了MRSA的毒素产生，并防止了MRSA对体外培养的角质形成细胞的损伤。总而言之，我们的结果表明，加拿大海参叶提取物含有多种成分的混合物，这些成分通过几种不同的机制对MRSA起作用。这些发现支持了加拿大毛细管藻粗提物在预防感染治疗中的传统应用。
The popular herbal remedy goldenseal (Hydrastis canadensis L.) is traditionally used to treat skin infections. With this study, we show activity of H. canadensis extracts in vitro against methicillin-resistant Staphylococcus aureus (MRSA). An extract from H. canadensis leaves demonstrated more potent antimicrobial activity than the alkaloid berberine alone (MICs of 75 µg/mL and 150 µg/mL, respectively). LC-MS detected alkaloids and efflux-pump inhibitory flavonoids in the extract, and the latter may explain the enhanced efficacy of the extract compared to berberine alone. We also show evidence of anti-virulence activity as a second mechanism by which H. canadensis acts against S. aureus. The H. canadensis leaf extract (but not the isolated alkaloids berberine, hydrastine, and canadine) demonstrated quorum quenching activity against several clinically relevant MRSA isolates (USA300 strains). Our data suggest that this occurs by attenuation of signal transduction through the AgrCA two-component system. Consistent with this observation, the extract inhibited toxin production by MRSA, and prevented damage by MRSA to keratinocyte cells in vitro. Collectively, our results show that H. canadensis leaf extracts possess a mixture of constituents that act against MRSA via several different mechanisms. These findings lend support for the traditional application of crude H. canadensis extracts in the treatment of prevention of infection.