Phenotype and function of hematopoietic stem cells.
Phenotype and function of hematopoietic stem cells.
复制标题
造血干细胞的表型和功能。
DOI：
10.1111/j.1749-6632.2001.tb03589.x
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发表时间：
2001
影响因子：
5.2
通讯作者：
Collector,MI
中科院分区：
文献类型：
--
作者：
Sharkis,SJ;Neutzel,S;Collector,MI
摘要：使用ANIN活体选择技术，我们可以分离出单个细胞，这些细胞可以重新填充致命性照射的小鼠受者的造血系统。这些细胞在动物体内迅速获得CD34表型。我们长期嵌合体中的祖先是捐赠者类型的。我们也有证据表明，具有这种长期再生能力(LTRA)的有限数量的移植(低至单个细胞)可以自我更新。移植后11个月移植到新宿主的连续4个月的骨髓移植证明了这一点。
Abstract:Using anin vivoselection technique, we can isolate individual cells that can repopulate the hematopoietic system of a lethally irradiated murine recipient. These cells rapidly acquire a CD34 phenotype in the animal. Progenitors in our long‐term chimeras are of donor type. We also have evidence that transplantation of limiting numbers (as low as a single cell) that have this long‐term repopulating ability (LTRA) can self‐renew. This is demonstrated by serial transplantation of marrow from engrafted recipients 11 months post transplant into new hosts for four additional months.