Increased CXCR3 Expression of Infiltrating Plasma Cells in Hunner Type Interstitial Cystitis.
Increased CXCR3 Expression of Infiltrating Plasma Cells in Hunner Type Interstitial Cystitis.
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DOI：
10.1038/srep28652
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发表时间：
2016-06-24
影响因子：
4.6
通讯作者：
Homma Y
中科院分区：
文献类型：
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作者：
Akiyama Y;Morikawa T;Maeda D;Shintani Y;Niimi A;Nomiya A;Nakayama A;Igawa Y;Fukayama M;Homma Y
CXCR3 通路上调和丰富的浆细胞浸润是亨纳型间质性膀胱炎 (HIC) 的特征。我们使用取自 27 名 HIC 患者和 15 名非 IC 膀胱炎患者的膀胱活检样本作为对照，进一步检查了这两个特征。通过数字图像分析对CD3阳性T淋巴细胞、CD20阳性B淋巴细胞、CD138阳性浆细胞和CXCR3阳性细胞的数量进行定量。采用CXCR3和CD138双免疫荧光法检测浆细胞中CXCR3的表达。探讨了 CXCR3 阳性与淋巴细胞和浆细胞数量以及临床参数之间的相关性。 HIC 和非 IC 膀胱炎标本中 CXCR3 阳性细胞的密度没有显着差异。然而，CXCR3 阳性在浆细胞中的分布表明 CXCR3 与 CD138 在 HIC 标本中共定位，但在非 IC 膀胱炎标本中则不然。 CXCR3 阳性细胞的数量与 HIC 样本中的浆细胞数量相关。 CXCR3 阳性细胞的浸润与 HIC 患者的临床参数无关。这些结果表明CXCR3阳性浆细胞的浸润是HIC的特征。 CXCR3 通路和特异性免疫反应可能参与浆细胞的积累/保留和 HIC 膀胱的病理生理学。
An up-regulated CXCR3 pathway and affluent plasma cell infiltration are characteristic features of Hunner type interstitial cystitis (HIC). We further examined these two features using bladder biopsy samples taken from 27 patients with HIC and 15 patients with non-IC cystitis as a control. The number of CD3-positive T lymphocytes, CD20-positive B lymphocytes, CD138-positive plasma cells, and CXCR3-positive cells was quantified by digital image analysis. Double-immunofluorescence for CXCR3 and CD138 was used to detect CXCR3 expression in plasma cells. Correlations between CXCR3 positivity and lymphocytic and plasma cell numbers and clinical parameters were explored. The density of CXCR3-positive cells showed no significant differences between HIC and non-IC cystitis specimens. However, distribution of CXCR3-positivity in plasma cells indicated co-localization of CXCR3 with CD138 in HIC specimens, but not in non-IC cystitis specimens. The number of CXCR3-positive cells correlated with plasma cells in HIC specimens alone. Infiltration of CXCR3-positive cells was unrelated to clinical parameters of patients with HIC. These results suggest that infiltration of CXCR3-positive plasma cells is a characteristic feature of HIC. The CXCR3 pathway and specific immune responses may be involved in accumulation/retention of plasma cells and pathophysiology of the HIC bladder.