ASSEMBLY OF VIRAL PARTICLES IN XENOPUS OOCYTES - PRE-SURFACE-ANTIGENS REGULATE SECRETION OF THE HEPATITIS-B VIRAL SURFACE ENVELOPE PARTICLE
ASSEMBLY OF VIRAL PARTICLES IN XENOPUS OOCYTES - PRE-SURFACE-ANTIGENS REGULATE SECRETION OF THE HEPATITIS-B VIRAL SURFACE ENVELOPE PARTICLE
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DOI：
10.1073/pnas.83.24.9338
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发表时间：
1986-12-01
影响因子：
11.1
通讯作者：
RUTTER, WJ
中科院分区：
文献类型：
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作者：
STANDRING, DN;OU, JH;RUTTER, WJ
乙型肝炎病毒 (HBV) 感染与病毒包膜颗粒的产生有关，该病毒包膜颗粒含有膜脂、表面抗原 (S) 和两个前表面抗原 (pre-S)，该抗原由整个 S 部分以及大约 55 个（前 S2）和 174 个（前 S1）附加 NH2 末端氨基酸组成。我们在这里展示了注射了合成 S mRNA 的爪蟾卵母细胞组装并分泌特征性的 22 nm 病毒包膜颗粒。相反，前S1和前S2抗原是合成的，但不分泌。通过共注射 mRNA，我们发现高水平前 S 蛋白的合成特异性抑制 S 抗原分泌。另一方面，高水平的 S 合成可以驱动少量任一前 S 抗原的分泌。这些观察结果与病毒包膜组装模型一致，其中 S 和前 S 蛋白可能通过 S 蛋白结构域之间的相互作用并入多聚体颗粒中，而前 S 氨基末端部分调节该结构的分泌。我们的结果表明，非洲爪蟾卵母细胞将为研究病毒或细胞起源的简单结构的形态发生提供强大的系统。
Infection with hepatitis B virus (HBV) is associated with the production of a viral envelope particle that contains membrane lipids, surface antigen (S), and two pre-surface-antigens (pre-S) comprised of the entire S moiety with approximately 55 (pre-S2) and 174 (pre-S1) additional NH2-terminal amino acids. We show here that Xenopus oocytes injected with synthetic S mRNA assemble and secrete characteristic 22-nm viral envelope particles. In contrast, pre-S1 and pre-S2 antigens are synthesized but not secreted. By coinjecting mRNAs, we found that synthesis of high levels of pre-S proteins specifically inhibits S antigen secretion. On the other hand, high levels of S synthesis can drive the secretion of small amounts of either pre-S antigen. These observations are consistent with a model for viral envelope assembly in which both S and pre-S proteins are incorporated into a multimeric particle, presumably via interactions between the S protein domains, while the pre-S amino-terminal moieties regulate the secretion of this structure. Our results indicate that Xenopus oocytes will provide a powerful system for studying the morphogenesis of simple structures of viral or cellular origin.