A novel Siglec-F+ neutrophil subset in the mouse nasal mucosa exhibits an activated phenotype and is increased in an allergic rhinitis model
A novel Siglec-F+ neutrophil subset in the mouse nasal mucosa exhibits an activated phenotype and is increased in an allergic rhinitis model
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DOI：
10.1016/j.bbrc.2020.03.122
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发表时间：
2020-06-04
影响因子：
3.1
通讯作者：
Hirata, Takako
中科院分区：
文献类型：
--
作者：
Matsui, Makoto;Nagakubo, Daisuke;Hirata, Takako
中性粒细胞是宿主防御病原体的重要吞噬细胞。它们被迅速招募到感染部位，释放抗菌肽和细胞因子，并吞噬和杀死微生物。中性粒细胞也在过敏性炎症部位聚集。在这里，我们使用小鼠过敏性鼻炎模型，其中小鼠腹腔注射卵清蛋白（OVA）致敏，然后通过鼻内给药的OVA或PBS的挑战，在鼻粘膜中的中性粒细胞积累的特点。在PBS-和OVA-激发小鼠的鼻粘膜中，我们发现在Ly-6 G（+）中性粒细胞群体中表达嗜酸性粒细胞标记物Siglec-F的细胞亚群。分选的Ly 6 G（+）Siglec-F+细胞的形态学分析显示，它们在胞质溶胶中缺乏嗜酸性颗粒，并且显然是嗜中性粒细胞，但与传统的Ly 6 G（+）Siglec-F-嗜中性粒细胞相比，它们具有更多分叶状的“葡萄状”核。在鼻咽相关淋巴组织、颈部淋巴结、脾脏或血液中几乎未发现Siglec-F+中性粒细胞。Siglec-F+嗜中性粒细胞和常规嗜中性粒细胞在OVA激发的小鼠的鼻粘膜中均显示数量增加。与常规Siglec-F-中性粒细胞相比，Siglec-F+中性粒细胞表现出活化的表型和增强的效应子功能。综上所述，我们的研究结果将Siglec-F+中性粒细胞鉴定为具有特异性存在于鼻粘膜中的活化表型的新型中性粒细胞亚群。(C)2020爱思唯尔公司All rights reserved.
Neutrophils are important phagocytic cells for host defense against pathogens. They are rapidly recruited to the site of infection, release antimicrobial peptides and cytokines, and engulf and kill microbes. Neutrophils also accumulate in allergic inflammatory sites. Here we characterized neutrophil accumulation in the nasal mucosa using a mouse model of allergic rhinitis, in which mice were sensitized by intraperitoneal injection of ovalbumin (OVA) and then challenged by intranasal administration of OVA or PBS. In the nasal mucosa of both PBS- and OVA-challenged mice, we found a cell subset expressing the eosinophil marker Siglec-F in the Ly-6G(+) neutrophil population. Morphological analysis of the sorted Ly6G(+)Siglec-F+ cells revealed that they were devoid of eosinophilic granules in the cytosol and were apparently neutrophils, but compared to conventional Ly-6G(+)Siglec-F- neutrophils, they had a more lobulated, "botryoid" nucleus. Siglec-F+ neutrophils were barely found in the nasopharynx-associated lymphoid tissue, cervical lymph nodes, the spleen, or blood. Both Siglec-F+ neutrophils and conventional neutrophils showed increased numbers in the nasal mucosa of OVA-challenged mice. Compared to conventional Siglec-F- neutrophils, Siglec-F+ neutrophils exhibited an activated phenotype and enhanced effector functions. Taken together, our findings identify Siglec-F+ neutrophils as a novel neutrophil subset with an activated phenotype that resides specifically in the nasal mucosa. (C) 2020 Elsevier Inc. All rights reserved.