HUMAN TRANSFORMING GROWTH FACTOR-ALPHA - PRECURSOR STRUCTURE AND EXPRESSION IN ESCHERICHIA-COLI
HUMAN TRANSFORMING GROWTH FACTOR-ALPHA - PRECURSOR STRUCTURE AND EXPRESSION IN ESCHERICHIA-COLI
复制标题
DOI：
10.1016/0092-8674(84)90550-6
复制
发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
GOEDDEL, DV
中科院分区：
文献类型：
--
作者：
DERYNCK, R;ROBERTS, AB;GOEDDEL, DV
转化生长因子-α（TGF-α）由许多人类肿瘤分泌，并可诱导非转化细胞系的可逆转化。使用长的合成脱氧寡核苷酸作为杂交探针，分离编码TGF-α的一部分的外显子。从人类基因组DNA文库中利用该外显子作为探针，将来源于人肾细胞癌的细胞系鉴定为TGF-α的来源。mRNA。克隆的TGF-α。c[互补]DNA从使用来自该细胞系的RNA制备的cDNA文库中分离，并编码160个氨基酸的前体多肽。50个氨基酸的成熟TGF-α。通过在E.大肠杆菌结合表皮生长因子受体并诱导培养的正常哺乳动物细胞的锚定独立性。
Transforming growth factor-.alpha. (TGF-.alpha.) is secreted by many human tumors and can induce the reversible transformation of nontransformed cell lines. Using long synthetic deoxyoligonucleotides as hybridization probes one isolated an exon coding for a portion of TGF-.alpha. from a human genomic DNA library. Utilizing this exon as a probe, a cell line derived from a human renal cell carcinoma was identified as a source of TGF-.alpha. mRNA. A cloned TGF-.alpha. c[complementary]DNA was isolated from a cDNA library prepared using RNA from this cell line and encodes a precursor polypeptide of 160 amino acids. The 50 amino acid mature TGF-.alpha. produced by expression of the appropriate coding sequence in E. coli binds to the epidermal growth factor receptor and induces the anchorage independence of normal mammalian cells in culture.