Yeast UAA suppressors effective in psi+ strains: leucine-inserting suppressors.
Yeast UAA suppressors effective in psi+ strains: leucine-inserting suppressors.
复制标题
对 psi 菌株有效的酵母 UAA 抑制剂：亮氨酸插入抑制剂。
DOI：
10.1016/0022-2836(79)90272-9
复制
发表时间：
1979
影响因子：
5.6
通讯作者：
F. Sherman
中科院分区：
文献类型：
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作者：
B. Ono;J. Stewart;F. Sherman
我们先前已经报道了从含有增加某些抑制子的作用效率的α +非孟德尔决定子的酵母酵母属（Saccharomyces）的单倍体菌株中分离和表征UAA抑制子（Onoet al.，1979年）。大多数抑制子引起酪氨酸或丝氨酸的插入。相反，营养标记物的抑制模式表明，罕见的抑制因子SUP 26插入了酪氨酸或丝氨酸以外的氨基酸。在这项研究中，我们报告了在缺乏尿嘧啶和含有刀豆氨酸的培养基上分离的类似于SUP 26的其他抑制子的特征;这种培养基预期排除丝氨酸插入抑制子，因为它们不抑制ura 4 - 1标记，并且排除酪氨酸插入抑制子，因为它们抑制can 1 - 100标记。对155个与SUP 26抑制基因相似的回复突变体进行了遗传学分析，这些回复突变体可以归属于6个不同的位点SUP 26、SUP 27、SUP 28、SUP 29、SUP 32和SUP 33中的一个或另一个。SUP 26、SUP 27和SUP 29位点分别定位在第XII、IV和X染色体上。SUP 29抑制基因的详细图谱位置提示它可能与霍桑和莫蒂默（1968）报道的SUP 30抑制基因等位。这6个抑制因子对UAA营养标记物的抑制模式相同，并且它们对异-1-细胞色素突变cyc 1 -72的作用效率都相似。每个这些抑制基因的效率增加了一个染色体异源抑制基因，萨尔。这6个抑制子都能使cyc 1 - 72突变的UAA位点的异1-细胞色素插入亮氨酸。这表明这些抑制因子的基因产物是相同的亮氨酸转移RNA的冗余形式。
We have previously reported the isolation and characterization of UAA suppressors from a haploid strain of yeastSaccharomyces cerevisiaecontaining theψ+non-Mendelian determinant which increases the efficiency of action of certain suppressors (Onoet al., 1979). Most of the suppressors caused the insertion of either tyrosine or serine. In contrast, the pattern of suppression of nutritional markers suggested that the rare suppressor,SUP26, inserted in an amino acid other than tyrosine or serine. In this investigation we report the characterization of additional suppressors, similar toSUP26, that were isolated on a medium lacking uracil and containing canavanine; this medium is expected to exclude serine-inserting suppressors because they do not suppress theura4-1marker, and to exclude tyrosine-inserting suppressors because they suppress thecan1-100marker. The total of 155 revertants similar to theSUP26suppressor were analyzed genetically and these could be assigned to one or another of the six distinct lociSUP26,SUP27,SUP28,SUP29,SUP32andSUP33. TheSUP26,SUP27andSUP29 loci mapped on chromosomes XII, IV and X, respectively. The detailed map position of theSUP29suppressor suggests that it may be allelic to theSUP30suppressor reported by Hawthorne & Mortimer (1968). These six suppressors had the same pattern of suppression of UAA nutritional markers and all of them had a similar low efficiency of action on the iso-1-cytochromecmutationcyc1-72. The efficiency of each of these suppressors was increased by a chromosomal allo-suppressor,sal. Each of the six suppressors caused the insertion of leucine in iso-1-cytochromecat the UAA site of thecyc1-72mutation. It is suggested that the gene products of these suppressors are redundant forms of the same leucine transfer RNA.