The cobalamin-binding proteins transcobalamin and haptocorrin in maternal and cord blood sera at birth
The cobalamin-binding proteins transcobalamin and haptocorrin in maternal and cord blood sera at birth
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DOI：
10.1373/clinchem.2005.057810
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发表时间：
2006-02-01
影响因子：
9.3
通讯作者：
Herrmann, W
中科院分区：
文献类型：
--
作者：
Obeid, R;Morkbak, AL;Herrmann, W
背景：血浆中有两种蛋白质携带维生素B-12。转钴胺素（TC）携带的维生素约占血浆总维生素B的25%，并有6%至20%的钴胺素饱和。Haptocorrin（HC）结合类似于80%的总钴胺素，并在很大程度上饱和与bobalamin.Methods：我们调查了分布和浓度之间的关系钴胺素，总和全息形式的TC，HC在血液样本中的孕妇分娩前（n = 92）和脐带血样本从他们的新生儿。我们还研究了这些蛋白质与维生素B-12状态的功能标志物甲基丙二酸（MMA）浓度之间的关系。结果：脐带血中总钴胺素、总HC、holoHC浓度和HC饱和度百分比均高于母血（平均钴胺素，268 vs 188 pmol/L;总HC，648 vs 538 pmol/L;全HC，441 vs 237 pmol/L; HC饱和度，70% vs 47%）。此外，脐带血中的总TC较低，而脐带血中的总TC和TC饱和度均高于母血（平均总TC，654 vs 1002 pmol/L;总TC，118 vs 53 pmol/L; TC饱和度，19.8% vs 5.4%）。母体血清钴胺素水平越高，脐血总胆固醇和总胆固醇饱和度越高（P < 0.05）。孕龄也是一个显着的决定因素，婴儿总TC，TC饱和度，总HC，和holoHC.Conclusion：之间的密切相关性的量holoTC存在于脐带血和母体血清中支持的重要性，母体钴胺素的状态，以确保足够的供应给婴儿。(c)2006年美国临床化学协会
Background: Two proteins carry vitamin B-12 in plasma. Transcobalamin (TC) carries similar to 25% of total plasma vitamin B,, and is 6% to 20% saturated with cobalamin. Haptocorrin (HC) binds similar to 80% of total cobalamin and is largely saturated with cobalamin.Methods: We investigated the distribution and the relationship between concentrations of cobalamin, total and holo forms of TC, and HC in blood samples from pregnant women just before delivery (n = 92) and in cord blood samples from their newborn babies. We also investigated the relationship between these proteins and concentrations of methylmalonic acid (MMA), the functional marker of vitamin B-12 status.Results: Concentrations of total serum cobalamin, total HC, holoHC, and percentage of HC saturation were higher in cord blood than in the maternal blood (mean cobalamin, 268 vs 188 pmol/L; total HC, 648 vs 538 pmol/L; holoHC, 441 vs 237 pmol/L; HC saturation, 70% vs 47%). Moreover, total TC was low in cord blood, whereas both holoTC and TC saturation were higher in cord blood than in the maternal blood (mean total TC, 654 vs 1002 pmol/L; holoTC, 118 vs 53 pmol/L; TC saturation, 19.8% vs 5.4%). Higher maternal serum cobalamin was associated with higher cord blood holoTC and TC saturation (P < 0.05). Gestational age was also a significant determinant of baby total TC, TC saturation, total HC, and holoHC.Conclusion: The close correlation between the amounts of holoTC present in cord blood and in maternal serum supports the importance of maternal cobalamin status for ensuring a sufficient supply to the baby. (c) 2006 American Association for Clinical Chemistry