Dielectric spectroscopy of red blood cells in sickle cell disease
Dielectric spectroscopy of red blood cells in sickle cell disease
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DOI：
10.1002/elps.202000143
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发表时间：
2021-02-03
期刊：
影响因子：
2.9
通讯作者：
Du, E.
中科院分区：
文献类型：
--
作者：
Liu, Jia;Qiang, Yuhao;Du, E.
缺氧诱导的镰状血红蛋白聚合和相关的离子跨细胞膜扩散可导致细胞介电性质的变化，其可潜在地用作镰状细胞疾病的无标记诊断生物标志物。本文提出了一种基于微流体的方法与片上气体控制的悬浮细胞的阻抗谱的频率范围内的40 Hz至110 MHz。镰状细胞在常氧和缺氧下的综合生物阻抗快速实现（在类似于7分钟内），并通过小样本体积（类似于2.5 μ L）进行分配。进行感测建模的分析，以获得镰状细胞悬浮液的介电谱和从测得的阻抗谱中提取单细胞特性的最佳条件。镰状细胞的研究结果表明，低氧处理后，细胞内部的介电常数和电导率增加，而细胞膜电容下降。此外，细胞介电参数的相对变化被发现是依赖于镰刀和胎儿血红蛋白水平。相比之下，正常红细胞在缺氧和常氧状态之间的变化是不明显的。镰状细胞的研究结果可作为设计以细胞介电特性为输入参数的基于介电常数的细胞分选和电变形测试装置的参考。从细胞悬浮液的阻抗谱的单细胞的介电特性的证明方法可以潜在地应用于其他细胞类型和在不同的气体条件下。
Hypoxia-induced polymerization of sickle hemoglobin and the related ion diffusion across cell membrane can lead to changes in cell dielectric properties, which can potentially serve as label-free, diagnostic biomarkers for sickle cell disease. This article presents a microfluidic-based approach with on-chip gas control for the impedance spectroscopy of suspended cells within the frequency range of 40 Hz to 110 MHz. A comprehensive bioimpedance of sickle cells under both normoxia and hypoxia is achieved rapidly (within similar to 7 min) and is appropriated by small sample volumes (similar to 2.5 mu L). Analysis of the sensing modeling is performed to obtain optimum conditions for dielectric spectroscopy of sickle cell suspensions and for extraction of single cell properties from the measured impedance spectra. The results of sickle cells show that upon hypoxia treatment, cell interior permittivity and conductivity increase, while cell membrane capacitance decreases. Moreover, the relative changes in cell dielectric parameters are found to be dependent on the sickle and fetal hemoglobin levels. In contrast, the changes in normal red blood cells between the hypoxia and normoxia states are unnoticeable. The results of sickle cells may serve as a reference to design dielectrophoresis-based cell sorting and electrodeformation testing devices that require cell dielectric characteristics as input parameters. The demonstrated method for dielectric characterization of single cells from the impedance spectroscopy of cell suspensions can be potentially applied to other cell types and under varied gas conditions.