Selenium nanoparticles as anti-infective implant coatings for trauma orthopedics against methicillin-resistant Staphylococcus aureus and epidermidis: in vitro and in vivo assessment
Selenium nanoparticles as anti-infective implant coatings for trauma orthopedics against methicillin-resistant Staphylococcus aureus and epidermidis: in vitro and in vivo assessment
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DOI：
10.2147/ijn.s197737
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发表时间：
2019-01-01
影响因子：
8
通讯作者：
O'Connor, Andrea J.
中科院分区：
文献类型：
--
作者：
Atran, Phong;O'Brien-Simpson, Neil;O'Connor, Andrea J.
背景资料：细菌感染是骨科植入物创伤后常见而严重的并发症，常与广泛的软组织损伤和污染伤口有关。在这些感染的伤口中发现了多重耐药细菌，特别是在有多重创伤和长期留在重症监护病房的患者中。目的：本研究的目的是开发一种有效对抗耐药细菌的骨科植入物涂层。方法和结果：我们将纳米粒子通过表面诱导成核-沉积法在钛种植体上制备了30 - 70 nm的微量元素硒（Se）涂层，并研究了其对耐甲氧西林金黄色葡萄球菌（MRSA）和甲氧西林金黄色葡萄球菌（MRSA）等耐药菌的抗菌活性。耐药表皮葡萄球菌（MRSE）在体外和感染的股骨模型在大鼠。纳米颗粒在体外显示出在低至0.5ppm的浓度下具有抗微生物活性。纳米粒子涂层强烈抑制生物膜形成的植入物，并减少了周围组织中的活菌数接种植入物与生物膜形成剂量的bacterios.Conclusion：这项研究显示了硒纳米粒子涂层的概念证明作为一个潜在的抗感染屏障骨科医疗器械的设置中的污染与多重耐药细菌。它也代表了硒纳米颗粒涂层减少耐药性骨科植入物感染的少数（如果只有）体内评估之一。
Background: Bacterial infection is a common and serious complication in orthopedic implants following traumatic injury, which is often associated with extensive soft tissue damage and contaminated wounds. Multidrug-resistant bacteria have been found in these infected wounds, especially in patients who have multi trauma and prolonged stay in intensive care units. Purpose: The objective of this study was to develop a coating on orthopedic implants that is effective against drug-resistant bacteria.Methods and results: We applied nanoparticles (30-70 nm) of the trace element selenium (Se) as a coating through surface-induced nucleation-deposition on titanium implants and investigated the antimicrobial activity against drug resistant bacteria including Methicillinresistant Staphylococcus aureus (MRSA) and Methicillin-resistant Staphylococcus epidermidis (MRSE) in vitro and in an infected femur model in rats. The nanoparticles were shown in vitro to have antimicrobial activity at concentrations as low as 0.5 ppm. The nanoparticle coatings strongly inhibited biofilm formation on the implants and reduced the number of viable bacteria in the surrounding tissue following inoculation of implants with biofilm forming doses of bacteria.Conclusion: This study shows a proof of concept for a selenium nanoparticle coatings as a potential anti-infective barrier for orthopedic medical devices in the setting of contamination with multi-resistant bacteria. It also represents one of the few (if only) in vivo assessment of selenium nanoparticle coatings on reducing antibiotic-resistant orthopedic implant infections.