Refinements to rodent head fixation and fluid/food control for neuroscience
Refinements to rodent head fixation and fluid/food control for neuroscience
复制标题
神经科学中啮齿动物头部固定和液体/食物控制的改进
DOI：
10.1016/j.jneumeth.2022.109705
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发表时间：
2022-09-15
影响因子：
3
通讯作者：
Prescott, Mark J.
中科院分区：
文献类型：
--
作者：
Barkus, Chris;Bergmann, Caroline;Prescott, Mark J.
头部固定在小鼠身上的使用在研究中越来越普遍，它的使用最初仅限于感觉神经科学领域。头枕通常与液体控制相结合，而不是食物限制，以激励行为，但现在也用于约束和非约束动物。尽管如此，关于如何最好地利用这些技术来优化科学成果和动物福利的指导很少。本文总结了目前的做法，并提供了改善动物福利和数据质量的建议，这些建议是基于对社区的调查、文献综述、专家意见和由英国国家研究动物替代、改进和减少中心（NC3Rs）召集的一个国际工作组的实践经验。涵盖的主题包括头部固定手术和术后护理，约束的习惯，以及使用液体/食物控制来激励表现。我们还讨论了一些最近的发展，这些发展可能提供了从大量行为试验中收集数据的替代方法，而不需要限制。目的是为各级研究人员、动物护理人员和伦理委员会提供支持，以根据3r的改进原则改进程序和实践。
The use of head fixation in mice is increasingly common in research, its use having initially been restricted to the field of sensory neuroscience. Head restraint has often been combined with fluid control, rather than food re-striction, to motivate behaviour, but this too is now in use for both restrained and non-restrained animals. Despite this, there is little guidance on how best to employ these techniques to optimise both scientific outcomes and animal welfare. This article summarises current practices and provides recommendations to improve animal wellbeing and data quality, based on a survey of the community, literature reviews, and the expert opinion and practical experience of an international working group convened by the UK's National Centre for the Replace-ment, Refinement and Reduction of Animals in Research (NC3Rs). Topics covered include head fixation surgery and post-operative care, habituation to restraint, and the use of fluid/food control to motivate performance. We also discuss some recent developments that may offer alternative ways to collect data from large numbers of behavioural trials without the need for restraint. The aim is to provide support for researchers at all levels, animal care staff, and ethics committees to refine procedures and practices in line with the refinement principle of the 3Rs.