THE LACTATE/PYRUVATE SHUTTLE IN SPERMATOZOA - OPERATION IN-VITRO
THE LACTATE/PYRUVATE SHUTTLE IN SPERMATOZOA - OPERATION IN-VITRO
复制标题
DOI：
10.1006/abbi.1994.1072
复制
发表时间：
1994-02-01
影响因子：
3.9
通讯作者：
BLANCO, A
中科院分区：
文献类型：
--
作者：
GALLINA, FG;DEBURGOS, NMG;BLANCO, A
在小鼠、大鼠和兔STM的体外重建系统中，研究了穿梭机的操作，用于在精子中间段中存在的特殊线粒体（精子型线粒体，STM）中转移还原当量。氧化还原对乳酸/丙酮酸和乳酸脱氢酶同工酶C-4参与穿梭。它对大鼠和兔STM有活性，而对小鼠STM不起作用，可能是因为乳酸盐流入小鼠细胞器相对较差。STM消耗丙酮酸/乳酸的比例分别为21.6，1.28和1.6小鼠，大鼠和兔的细胞器，分别。穿梭被0.6 mM mersalyl抑制，mersalyl是乳酸转运的阻断剂。穿梭机的操作将氧化在线粒体中具有有效乳酸载体的那些物种的精子中有氧糖酵解（或果糖酵解）期间产生的胞质NADH。(C)1994年出版社出版。
The operation of a shuttle for the transfer of reducing equivalents in the special mitochondria present in the middle piece of spermatozoa (sperm-type mitochondria, STM) was studied in reconstituted systems in vitro with mouse, rat, and rabbit STM. The redox couple lactate/ pyruvate and the lactate dehydrogenase isozyme C-4 are involved in the shuttle. It is active with rat and rabbit STM, while it does not work with mouse STM, probably because the influx of lactate into the mouse organelles is relatively poor. Ratios of consumption of pyruvate/lactate by STM were 21.6, 1.28, and 1.6 for mouse, rat, and rabbit organelles, respectively. The shuttle is inhibited by O.6 mM mersalyl, a blocker of lactate transport. The operation of the shuttle would oxidize cytosolic NADH produced during aerobic glycolysis (or fructolysis) in spermatozoa of those species having an efficient lactate carrier in mitochondria. (C) 1994 Academic Press,Inc.