The gene coding for protein p60 of Listeria monocytogenes and its use as a specific probe for Listeria monocytogenes
The gene coding for protein p60 of Listeria monocytogenes and its use as a specific probe for Listeria monocytogenes
复制标题
单核细胞增生李斯特菌蛋白p60的编码基因及其作为单核细胞增生李斯特菌特异性探针的应用
作者：
S. Köhler;M. Leimeister;T. Chakraborty;F. Lottspeich;W. Goebel
在大肠杆菌中克隆了单核增生李斯特菌编码一种主要细胞外蛋白（p60）的基因。该基因被命名为iap，因为p60先前被证明代表一种入侵相关蛋白（M. Kuhn和W. Goebel，《感染》）。免疫学杂志。57:55-61,1989)。免疫印迹显示重组大肠杆菌克隆表达p60。测定了iap的完整核苷酸序列。推断出的p60（484个氨基酸）的氨基酸序列包含27个氨基酸的n端信号序列和一个由19个苏氨酸-天冬酰胺单元组成的扩展重复区。与整个iap基因的杂交显示，在大多数其他李斯特菌种中存在同源序列。相比之下，包含整个重复区的400碱基对内部iap探针仅与单核增生L.的基因组DNA杂交。四种寡核苷酸先前被描述为检测单核增生李斯特菌的特异性探针(A. R. Datta， B. A. Wentz， D. Shook, and M. W. Trucksess, appll。环绕。微生物学杂志。54:2933-2937,1988)被证明是iap基因的一部分。
The gene of Listeria monocytogenes that encodes a major extracellular protein (p60) was cloned in Escherichia coli. The gene was designated iap, as p60 was previously shown to represent an invasion-associated protein (M. Kuhn and W. Goebel, Infect. Immun. 57:55-61, 1989). The recombinant E. coli clone expressed p60, as shown by immunoblotting. The complete nucleotide sequence of iap was determined. The deduced amino acid sequence of p60 (484 amino acids) contains a putative N-terminal signal sequence of 27 amino acids and an extended repeat region consisting of 19 threonine-asparagine units. Hybridization with the entire iap gene revealed the presence of homologous sequences in most other Listeria species. In contrast, a 400-base-pair internal iap probe which contained the whole repeat region hybridized only with genomic DNA from L. monocytogenes. Four oligonucleotides previously described as specific probes for the detection of L. monocytogenes (A. R. Datta, B. A. Wentz, D. Shook, and M. W. Trucksess, Appl. Environ. Microbiol. 54:2933-2937, 1988) were shown to be part of the iap gene.
球状蛋白质堆积的疏水基础。
DOI：
10.1073/pnas.77.8.4643
发表时间：
1980
影响因子：
11.1
作者：
Rose,GD;Roy,S
通讯作者：
Roy,S