Comparison of the indigenous oral microbiota and immunoglobulin responses of athymic (nu/nu) and euthymic (nu/(+)) mice
Comparison of the indigenous oral microbiota and immunoglobulin responses of athymic (nu/nu) and euthymic (nu/(+)) mice
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DOI：
10.1111/j.1399-302x.1997.tb00370.x
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发表时间：
1997-06-01
影响因子：
--
通讯作者：
Lavoie, MC
中科院分区：
文献类型：
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作者：
Marcotte, H;Lavoie, MC
免疫系统在本土口腔细菌种群的稳态中的作用知之甚少。在这项研究中，我们比较了特定病原体无胸腺裸（nu/nu）BALB/c小鼠与其相应的表型正常（nu/+）的同窝仔的土著口腔微生物群的演变。我们还评估了相应的唾液和血清抗体活性（伊加和IgG）对占主导地位的土著口腔细菌。从两种小鼠品系中回收的细菌物种为鼠乳杆菌。粪肠球菌、口腔链球菌和表皮葡萄球菌。从27日龄开始，nu/+和nu/nu小鼠的口腔细菌种群比例显著不同。当微生物群稳定时（在40日龄时），nu/+小鼠的总可培养微生物群以L. murinus（65-85%），而nu/nu小鼠则以E.粪菌（40-60%）。改变nu/nu小鼠口腔驻留微生物群的确切因素尚不清楚。我们发现，总唾液伊加水平显着降低nu/nu小鼠，但没有观察到之间的唾液伊加抗体水平对土著细菌和这些土著细菌在口腔微生物群的比例。裸鼠微生物群的变化可能是由其他因素引起的，如其他免疫功能缺陷或冷应激。
The role of the immune system in the homeostasis of indigenous oral bacterial populations is poorly understood. In this study, we compared the evolution of the indigenous oral microbiota of specific pathogen-free athymic nude (nu/nu) BALB/c mice with that of their corresponding phenotypically normal (nu/+) littermates. We also evaluated corresponding salivary and serum antibody activities (IgA and IgG) against the predominant indigenous oral bacteria. The bacterial species recovered from the two mouse strains were Lactobacillus murinus. Enterococcus faecalis, Streptococcus oralis and Staphylococcus epidermidis. From 27 days of age, nu/+ and nu/nu mice had significantly different proportions of oral bacterial populations. When the microbiota stabilized (at 40 days of age), the total cultivable microbiota of nu/+ mice was dominated by L. murinus (65-85%), while that of nu/nu mice was dominated by E. faecalis (40-60%). The precise factors that alter the oral resident microbiota in nu/nu mice are unknown. We found that total salivary IgA levels were significantly lower in nu/nu mice, but no association were observed between the level of salivary IgA antibody against indigenous bacteria and the proportion of these indigenous bacteria in the oral microbiota. The change in the microbiota of nude mice may have been caused by other factors such as defects in other immune functions or cold stress.