Alveolar epithelial cells: differentiation and lung injury
Alveolar epithelial cells: differentiation and lung injury
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DOI：
10.1111/j.1440-1843.2006.00804.x
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发表时间：
2006-01
期刊：
影响因子：
6.9
通讯作者：
K. Sugahara;J. Tokumine;K. Teruya;T. Oshiro
中科院分区：
文献类型：
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作者：
K. Sugahara;J. Tokumine;K. Teruya;T. Oshiro
摘要肺泡上皮细胞的再上皮化是多种类型肺损伤的重要修复过程之一。肺泡II型细胞的主要功能是合成和分泌表面活性剂，肺泡上皮损伤后增生，并作为肺泡I型细胞的祖细胞。作者检测了几种可溶性因子对体外培养肺泡II型细胞的影响，并检测了LPS、博来霉素和/或角化细胞生长因子处理大鼠肺中表面活性剂蛋白的组织病理学和基因表达。作者接下来研究了骨髓基质细胞（BMSC）经静脉植入博来霉素诱导的肺部的效果。作者发现，角化细胞生长因子（KGF）是肺泡II型细胞的强生长因子，KGF灌注可预防博来霉素诱导的肺损伤。此外，作者还展示了移植骨髓间充质干细胞向肺泡上皮细胞分化的可能性。KGF和BMSC可能在肺泡上皮的维持和损伤后受损上皮的修复中发挥重要作用，并可能提供潜在的治疗选择。
Abstract: Re‐epithelialization of alveolar epithelial cells is one of the important repair processes in many types of lung injury. The major functions of alveolar type II cells are synthesis and secretion of surfactant, hyperplasia in reaction to alveolar epithelial injury, and serving as progenitor cells for alveolar type I cells. The authors have examined the effects of several soluble factors on cultured alveolar type II cells in vitro, and also examined the histopathology and gene expression of surfactant proteins in the rat lungs with LPS, bleomycin and/or treated with keratinocyte growth factor. The authors next examined the effects of bone marrow stromal cells (BMSC) implanted transvenously into bleomycin‐induced lungs. The authors found that keratinocyte growth factor (KGF) is a strong growth factor for alveolar type II cells, and that KGF instillation prevents bleomycin‐induced lung injury. Furthermore, the authors showed the possibility of differentiation of implanted BMSC into alveolar epithelial cells. KGF and BMSC may play an important role in maintaining the alveolar epithelium and repairing the damaged epithelium after injury, and may well provide potential therapeutic alternatives.