Disrupting Il13 impairs production of IL-4 specified by the linked allele.
Disrupting Il13 impairs production of IL-4 specified by the linked allele.
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破坏 Il13 会损害相关等位基因指定的 IL-4 的产生。
DOI：
10.1038/87778
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发表时间：
2001
影响因子：
30.5
通讯作者：
Paul,WE
中科院分区：
文献类型：
--
作者：
Guo,L;Hu-Li,J;Zhu,J;Pannetier,C;Watson,C;McKenzie,GJ;McKenzie,AN;Paul,WE
白细胞介素13缺陷（IL-13-/-）小鼠在IL-4产生的引发中表达缺陷，该缺陷不能通过向引发培养物中添加IL-13来纠正。这部分是由于在IL-13−/− CD 4 + T细胞培养过程中消耗了IL-4而没有内源性替代。我们检查了小鼠的细胞，其中破坏的IL 13与一条染色体上的野生型IL 4相连，野生型IL 13与IL 4基因座中的“敲入”绿色荧光蛋白（GFP）基因相连。我们的研究结果表明，IL-4产生的赤字是由于，至少部分是由于顺式效应，其中破坏IL-13减少IL-4的生产从连接的IL-4基因。
Interleukin 13–deficient (IL-13−/−) mice express a defect in priming for IL-4 production that is not corrected by adding IL-13 to the priming culture. This is partly accounted for by the consumption of IL-4 without endogenous replacement during culture of IL-13−/− CD4+ T cells. We examined cells from mice in which disrupted Il13 was linked to wild-type Il4 on one chromosome and wild-type Il13 was linked to a “knocked-in” green fluorescent protein (Gfp) gene in the Il4 locus. Our results show that the deficit in IL-4 production was due, at least in part, to a cis effect, in which disrupted Il13 diminished IL-4 production from the linked Il4 gene.