Anti-inflammatory effect of aqueous extract from Kawachi-bankan (Citrus maxima) peel in vitro and in vivo
Anti-inflammatory effect of aqueous extract from Kawachi-bankan (Citrus maxima) peel in vitro and in vivo
复制标题
DOI：
10.1007/s10616-019-00323-4
复制
发表时间：
2019-08-01
期刊：
影响因子：
2.2
通讯作者：
Sugahara, Takuya
中科院分区：
文献类型：
--
作者：
Ishida, Momoko;Takekuni, Chihiro;Sugahara, Takuya
Kawachi-bankan（Citrus maxima）是日本爱媛县生产的柑橘之一。柑橘皮中的类黄酮和类胡萝卜素的保健功能已得到了较好的研究，但柑橘皮中的水溶性物质的保健功能尚未引起人们的重视。本文研究了川内木香皮水提物（KPE）的体内外抗炎作用。KPE显著抑制LPS刺激的RAW 264.7细胞产生炎性细胞因子，如白细胞介素（IL）-6和肿瘤坏死因子（TNF）-α，而无细胞毒性。KPE还显著抑制细胞中IL-6和TNF-α的mRNA表达水平，表明KPE通过抑制基因表达水平来抑制炎性细胞因子的产生。免疫印迹分析显示，KPE通过抑制p38磷酸化和NF-κ B B易位到细胞核中而对巨噬细胞显示出抗炎作用。口服KPE可抑制全身炎症反应综合征（SIRS）模型小鼠血清中炎性细胞因子的水平，提高小鼠的生存率。我们使用细胞系的实验表明，KPE抑制炎症状态下巨噬细胞产生炎症细胞因子。此外，体内实验表明，口服KPE可抑制SIRS模型小鼠血清炎症细胞因子水平，提高SIRS模型小鼠的生存率。我们的研究结果表明，KPE有助于减轻炎症反应。
Kawachi-bankan (Citrus maxima) is one of the citruses produced in Ehime, Japan. Although health functions of flavonoids and carotenoids in citrus peel have been studied very well, those of water-soluble substances in the peel have not been focused. We herein indicated the anti-inflammatory effect of Kawachi-bankan peel aqueous extract (KPE) in vitro and in vivo. KPE significantly inhibited the production of inflammatory cytokines such as interleukin (IL)-6 and tumor necrosis factor (TNF)-alpha by LPS-stimulated RAW264.7 cells without cytotoxicity. KPE also significantly inhibited the mRNA expression levels of IL-6 and TNF-alpha in the cells, suggesting that KPE inhibits the production of inflammatory cytokines by suppressing the gene expression levels. Immunoblot analysis revealed that KPE shows an anti-inflammatory effect on macrophages through the suppression of the phosphorylation of p38 and the translocation of NF-kappa B into nucleus. The oral administration of KPE inhibited the serum levels of inflammatory cytokines and improved the survival rate in systemic inflammatory response syndrome (SIRS) model mice. Our experiments using a cell line suggested that KPE inhibits the production of inflammatory cytokines by macrophages in hyperinflammatory state. In addition, experiments in vivo showed that the oral administration of KPE inhibited the serum levels of inflammatory cytokines and improved the survival rate in SIRS model mice. Our findings indicated that KPE contributes to alleviating of a hyperinflammatory response.