Spatiotemporally organized immunomodulatory response to SARS-CoV-2 virus in primary human broncho-alveolar epithelia.
Spatiotemporally organized immunomodulatory response to SARS-CoV-2 virus in primary human broncho-alveolar epithelia.
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DOI：
10.1016/j.isci.2023.107374
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发表时间：
2023-08-18
期刊：
影响因子：
5.8
通讯作者：
Palucka, Karolina
中科院分区：
文献类型：
--
作者：
Castaneda, Diana Cadena;Jangra, Sonia;Yurieva, Marina;Martinek, Jan;Callender, Megan;Coxe, Matthew;Choi, Angela;Diego, Juan Garcia-Bernalt;Lin, Jianan;Wu, Te-Chia;Marches, Florentina;Chaussabel, Damien;Yu, Peter;Salner, Andrew;Aucello, Gabrielle;Koff, Jonathan;Hudson, Briana;Church, Sarah E.;Gorman, Kara;Anguiano, Esperanza;Garcia-Sastre, Adolfo;Williams, Adam;Schotsaert, Michael;Palucka, Karolina
新冠肺炎大流行仍然是一场健康危机，有大量未得到满足的医疗需求。呼吸道上皮细胞是病毒调控向严重疾病发展的第一个靶点，其早期反应尚不完全清楚。采用原代培养的人肺泡上皮细胞气液界面培养物，研究SARS-CoV-2变异株感染的动力学和动力学。用核蛋白表达来衡量，武汉样病毒感染是在感染后4-6天出现的一个晚期事件。其他变种显示感染的时间线越来越快。所有的变异体都触发了相似的转录信号，即“早期”的炎症/免疫信号先于“晚期”的I/III型干扰素，但发现了质量和动力学上的差异，这与核蛋白表达的时间一致。对病毒的反应在空间上是有序的：CSF3在基底层细胞表达，CCL20在顶端细胞表达。因此，SARS-CoV-2病毒触发了随着时间的推移而调节的特定反应，以参与不同的免疫反应。SARS-CoV-2武汉样病毒及其变异株感染病毒在呼吸道上皮细胞中触发双向转录反应的动力学不同CSF3病毒诱导的上皮基底侧空间蛋白反应与CCL20上皮尖端侧病毒诱导的空间蛋白反应生物科学；分子生物学；免疫学；微生物学
The COVID-19 pandemic continues to be a health crisis with major unmet medical needs. The early responses from airway epithelial cells, the first target of the virus regulating the progression toward severe disease, are not fully understood. Primary human air-liquid interface cultures representing the broncho-alveolar epithelia were used to study the kinetics and dynamics of SARS-CoV-2 variants infection. The infection measured by nucleoprotein expression, was a late event appearing between day 4–6 post infection for Wuhan-like virus. Other variants demonstrated increasingly accelerated timelines of infection. All variants triggered similar transcriptional signatures, an “early” inflammatory/immune signature preceding a “late” type I/III IFN, but differences in the quality and kinetics were found, consistent with the timing of nucleoprotein expression. Response to virus was spatially organized: CSF3 expression in basal cells and CCL20 in apical cells. Thus, SARS-CoV-2 virus triggers specific responses modulated over time to engage different arms of immune response. SARS-CoV-2 Wuhan-like virus and variants displayed different kinetics of infection Virus triggers a biphasic & polarized transcriptional response in airway epithelia Virally induced spatial protein response with CSF3 on epithelial basal side Virally induced spatial protein response with CCL20 on epithelial apical side Biological sciences; Molecular biology; Immunology; Microbiology
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影响因子：
6.4
作者：
Boumaza, Asma;Gay, Laetitia;Mege, Jean-Louis
通讯作者：
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影响因子：
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作者：
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DOI：
10.1016/s0167-4889(00)00136-1
发表时间：
2001-02-05
影响因子：
5.1
作者：
Besnard, V;Corroyer, S;Clement, A
通讯作者：
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影响因子：
3.8
作者：
Chagula, Damian B.;Rechcinski, Tomasz;Chmiela, Magdalena
通讯作者：
Chmiela, Magdalena