Increase of HIV-1 subtype A in Central African Republic.
Increase of HIV-1 subtype A in Central African Republic.
复制标题
中非共和国 HIV-1 A 亚型的增加。
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
S. Saragosti
中科院分区：
文献类型：
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作者：
M. Müller;M. Chaix;F. Letourneur;E. Bégaud;D. Beaumont;A. Deslandres;B. You;J. Morvan;C. Mathiot;F. Barré;S. Saragosti
据报告，在中非共和国班吉同时存在五种不同的HIV-1亚型和未分类的HIV-1。在1990年到1991年之间。先前的研究是在属于C.A.R.的个体中进行的。武装部队（FACA）队列和来自包吉大学医院的患者。为了跟踪班吉HIV-1亚型随时间的分布，我们在1987年至1997年期间对班吉的三组个体进行了HIV-1亚型的横断面监测：47名属于FACA队列的男性，38名来自CNHUB医院的患者和51名咨询性传播疾病（STD）诊所的个体。通过异源双链迁移率测定（HMA）和/或对包含V3结构域的env区进行测序，对110例HIV-1进行亚型分型。通过比较两个时间段（1987-1991年和1991-1996年）在FACA队列中的HIV-1分布，我们观察到A亚型从43.7%显著增加到83.9%。这种亚型分布似乎并不特异于FACA队列，因为在研究的第一个时间段，A亚型占CNHUB患者HIV-1感染的46.7%，在第二个时间段占69.6%。1995年和1997年性病患者中A亚型感染率分别为72.7%和89.7%。在第二个时间段可以鉴定出E亚型病毒，但在所研究的三组个体中仅占感染的6.5%至21.8%。其他亚型（B、C、H）和C2-V3中未分类的HIV-1在第二个时间段中检出的频率仅为3.2%至9.1%。系统发育分析排除了研究中所包括个体的单一来源感染。我们的数据表明，艾滋病毒-1亚型A感染在班吉的比例增加，提出了一个特定的艾滋病毒-1变种的优先传播的问题。
The concomitant presence of five distinct HIV-1 subtypes and of unclassified HIV-1 was reported in Bangui, Central African Republic (C.A.R.) between 1990 and 1991. This previous study was conducted in individuals belonging to the C.A.R. Armed Forces (FACA) Cohort and in patients from the University Hospital of Baugui. To follow the HIV-1 subtype distribution in Bangui over time, we conducted a cross-sectional surveillance of HIV-1 subtypes between 1987 and 1997 in three groups of individuals in Bangui: 47 men belonging to the FACA Cohort, 38 patients from the CNHUB hospital, and 51 individuals consulting the sexually transmitted diseases (STD) clinic. One hundred and ten HIV-1 were subtyped by heteroduplex mobility assay (HMA) and/or sequencing of env regions encompassing the V3 domain. By comparing the HIV-1 distribution in two time periods (1987-1991 and 1991-1996) in the FACA cohort, we observed a significant increase of subtype A from 43.7% to 83.9%. This subtype distribution does not seem specific to the FACA cohort, in that subtype A accounted for 46.7% of the HIV-1 infections in CNHUB patients in the first time period studied and for 69.6% in the second time period. In STD patients, subtype A infections were predominant in 1995 (72.7%) and 1997 (89.7%). Subtype E viruses could be identified in the second time period, but represented only between 6.5% and 21.8% of the infections in the three groups of individuals studied. Other subtypes (B, C, H) and non-classified HIV-1 in C2-V3 were detected with only a 3.2% to 9.1% frequency for each in the second time period. Phylogenetic analysis excluded infection by a single source for the individuals included in the study. Our data demonstrate an increase in the proportion of HIV-1 subtype A infections in Bangui that raises the question of a preferential transmissibility of specific HIV-1 variants.