Development of a multiplex PCR assay to discriminate native Hymenoscyphus albidus and introduced Hymenoscyphus fraxineus in Britain and assess their distribution
Development of a multiplex PCR assay to discriminate native Hymenoscyphus albidus and introduced Hymenoscyphus fraxineus in Britain and assess their distribution
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DOI：
10.1016/j.funeco.2016.06.002
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发表时间：
2016-10-01
期刊：
影响因子：
2.9
通讯作者：
Webber, Joan F.
中科院分区：
文献类型：
--
作者：
King, Kevin M.;Webber, Joan F.
在欧洲有两种与白蜡树（白蜡树属）有关的Hymenoscyphus;最近到达的白蜡树枯死病原体Hymenoscyphus fraxineus和显然是本地的Hymenoscyphus albidus。然而，在欧洲部分地区，入侵的H. Fraxineus似乎正在取代H。因此可能面临灭绝。我们根据calmodulih序列设计引物，用于单重和多重PCR检测，以检测和区分这两种形态上非常相似的物种。对50个分离株和26个田间采后样品进行了检测。albidus/H.来自欧洲和亚洲的Fraxineus，加上Hymenoscyphus frustigenus、Hymenoscyphus koreanus和Hymenoscyphus occultus的分离物。使用该测定法证实了H. albidus和H. 2014年至2015年间，fraxineus在英国南部出现，H.在八个地理位置不同的地点发现了白纹伊蚊。本文还讨论了该方法的敏感性、特异性和潜在的应用前景。皇冠版权所有（C）2016由爱思唯尔有限公司出版。保留所有权利。
Two Hymenoscyphus species are associated with ash (Fraxinus) in Europe; the recently arrived ash dieback pathogen Hymenoscyphus fraxineus and apparently native Hymenoscyphus albidus. However, in parts of Europe invasive H. fraxineus appears to be replacing H. albidus which may, therefore, face extinction. We designed primers based on calmodulih sequences for use in singleplex and multiplex PCR assays to detect and discriminate between these two morphologically very similar species. The multiplex assay was tested against 50 isolates and 26 apothecial field samples of H. albidus/H. fraxineus from Europe and Asia, plus isolates of Hymenoscyphus fructigenus, Hymenoscyphus koreanus and Hymenoscyphus occultus. Use of the assay confirmed both H. albidus and H. fraxineus were present across southern Britain between 2014 and 2015, with H. albidus detected at eight geographically distinct sites. The sensitivity, specificity and potential applications of the multiplek assay are discussed. Crown Copyright (C) 2016 Published by Elsevier Ltd. All rights reserved.