Expression of ERalpha, ERbeta and co-regulator PELP1/MNAR in colorectal cancer: prognostic significance and clinicopathologic correlations.
Expression of ERalpha, ERbeta and co-regulator PELP1/MNAR in colorectal cancer: prognostic significance and clinicopathologic correlations.
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DOI：
10.3233/clo-2009-0467
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Kalofonos H
中科院分区：
文献类型：
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作者：
Grivas PD;Tzelepi V;Sotiropoulou-Bonikou G;Kefalopoulou Z;Papavassiliou AG;Kalofonos H
背景：雌激素受体β（ERβ）在结直肠组织中大量表达，但其在结直肠癌发生中的作用仍不清楚。 ER 新型协同调节因子富含脯氨酸、谷氨酸和亮氨酸的蛋白 1 (PELP1/MNAR) 已得到表征，但尚未研究其在结直肠癌中的表达。 方法：采用免疫组织化学方法检测113例结直肠癌患者的结直肠正常粘膜、腺瘤和腺癌中ERα、ERβ和PELP1/MNAR蛋白的表达情况。 结果：ERα在结直肠组织中表达极为罕见，其表达似乎与结直肠癌发生无关。在上皮细胞、内皮细胞、炎症细胞、平滑肌细胞和肌成纤维细胞的细胞核中检测到 ERβ 和 PELP1/MNAR。当考虑染色强度时，与正常粘膜相比，癌上皮细胞中两种蛋白的表达均显着增加。上皮细胞中的 ERβ 表达与疾病无进展生存率降低相关。发现上皮细胞中 PELP1/MNAR 过度表达是一个独立的有利预后因素。此外，与腺瘤和正常粘膜相比，两种蛋白的表达在癌的基质肌成纤维细胞中显着增加。 结论：ERβ和PELP1/MNAR似乎参与结直肠肿瘤的发生，并可能具有预后意义。
Background: Estrogen receptor β (ERβ) is abundantly expressed in colorectal tissue, but its role in colorectal carcinogenesis remains elusive. ER novel co-regulator, proline-, glutamic acid- and leucine-rich protein 1 (PELP1/MNAR) has been characterized, but its expression in colorectal carcinomas has not been investigated. Methods: ERα, ERβ and PELP1/MNAR protein expression were evaluated by immunohistochemistry in colorectal normal mucosa, adenomas and adenocarcinomas from 113 patients with colorectal cancer. Results: ERα expression is extremely rare in colorectal tissue and its expression does not appear to be associated with colorectal carcinogenesis. ERβ and PELP1/MNAR were detected in the nucleus of epithelial, endothelial, inflammatory, smooth muscle cells and myofibroblasts. When intensity of staining was taken into account, the expression of both proteins was significantly increased in epithelial cells of carcinomas compared to normal mucosa. ERβ expression in epithelial cells was correlated with decreased disease progression – free survival. PELP1/MNAR overexpression in epithelial cells was found to be an independent favorable prognostic factor. Additionally, the expression of both proteins was significantly increased in stromal myofibroblasts of carcinomas compared to adenomas and normal mucosa. Conclusion: ERβ and PELP1/MNAR appear to be involved in colorectal tumorigenesis and might have prognostic significance.