Long-term consequences of early maternal deprivation in serotonergic activity and HPA function in adult rat
Long-term consequences of early maternal deprivation in serotonergic activity and HPA function in adult rat
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DOI：
10.1016/j.neulet.2010.04.054
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发表时间：
2010-08-09
影响因子：
2.5
通讯作者：
Konstandi, Maria
中科院分区：
文献类型：
--
作者：
Rentesi, Georgia;Antoniou, Katerina;Konstandi, Maria
越来越多的证据表明，早期生活压力事件可能会导致神经发育的永久性改变，这反过来又可能导致成年后精神病理学的发展。特别是，产妇剥夺（MD）在大鼠24小时已与几个异常的大脑和行为在成年期，相关的焦虑症的神经生物学基板。本研究的目的是澄清长期影响MD，下丘脑-垂体-肾上腺（HPA）轴的活动和肾上腺素能（5-HT）的功能，在成年期，尚未得到彻底调查的科目。为了这个目的。在出生后第9天（pnd 9），将Wistar大鼠幼仔从其母亲身边剥夺24小时，并在69-90天时进行检查。血浆皮质酮和ACTH水平沿着动物在开阔场地的行为被用作应激指标。此外，估计下丘脑和海马中的多巴胺能活性，这是协调神经内分泌和对压力的行为反应的关键结构。有趣的是，在成年期，与对照组相比，MD大鼠显示体重减轻，肾上腺素能活动和“焦虑”相关行为增加，以及血浆皮质酮和ACTH水平升高。这项研究的结果表明，MD导致HPA轴和肾上腺素能活动的长期改变，表明早期生活压力事件与成年后焦虑样障碍的发展之间存在明确的关系。(C)2010爱思唯尔爱尔兰有限公司版权所有。
Increasing body of evidence indicates that early life stressful events may induce permanent alterations in neurodevelopment, which in turn, could lead to the development of psychopathologies in adulthood. In particular, maternal deprivation (MD) for 24 h in rats has been associated with several abnormalities in brain and behaviour during adulthood, relevant to the neurobiological substrate of anxiety disorders. The aim of the present study was to clarify the long-term effects of MD, on hypothalamo-pituitary-adrenal (HPA) axis activity and serotonergic (5-HT) function, in adulthood, subjects that have not been yet thoroughly investigated. For this purpose. Wistar rat pups were deprived from their mothers for a 24-h single period at postnatal day 9 (pnd 9) and were examined when aged 69-90 days. Plasma corticosterone and ACTH levels along with the animal's behaviour in an open field were used as indices of stress. Moreover, serotonergic activity was estimated in hypothalamus and hippocampus, key structures in the coordination of neuroendocrine and behavioural responses to stress. Interestingly, in adulthood, MD rats compared to controls, displayed decreased body weight, increased serotonergic activity and "anxiety" related behaviour, as well as elevated plasma corticosterone and ACTH levels. The findings of this study showed that MD results in long-term modifications in HPA axis and serotonergic activity indicating a clear relationship between early life stressful events and the development of anxiety-like disorders later in adulthood. (C) 2010 Elsevier Ireland Ltd. All rights reserved.