Linking isoprenoidal GDGT membrane lipid distributions with gene abundances of ammonia-oxidizing Thaumarchaeota and uncultured crenarchaeotal groups in the water column of a tropical lake (Lake Challa, East Africa)
Linking isoprenoidal GDGT membrane lipid distributions with gene abundances of ammonia-oxidizing Thaumarchaeota and uncultured crenarchaeotal groups in the water column of a tropical lake (Lake Challa, East Africa)
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DOI：
10.1111/1462-2920.12118
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发表时间：
2013-09-01
影响因子：
5.1
通讯作者：
Damste, Jaap S. Sinninghe
中科院分区：
文献类型：
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作者：
Buckles, Laura K.;Villanueva, Laura;Damste, Jaap S. Sinninghe
层状湖泊是微生物多样性的重要储集层，为古细菌的生态位分化提供了栖息地。在这项研究中，我们使用了一种基于脂质生物标志物/ dna的方法来揭示查拉湖（东非）水柱中古细菌的多样性和丰度。完整极性脂质（IPL） crenarchaeol的浓度（1ng l(-1)）在氧跃层/硝基跃层处增加。较不稳定的IPL己糖-磷酸己糖crenarchaeol的优势表明存在活跃的Thaumarchaeota群落。古细菌16S rRNA克隆文库显示，在氧基层及以上存在1.1a和1.1b古细菌群。在缺氧的深水中，amoA基因丰度比氧层低一个数量级，与无环甘油二烷基甘油四醚（GDGT-0）的IPL明显具有高丰度（约为90ng l(-1)）。未培养的crenarchaeota群体1.2和杂crenarchaeota群体（MCG）的古细菌16S rRNA序列的优势表明GDGT-0起源于未培养的crenarchaeota。本研究证明了热分层和养分有效性在古菌群分布中的重要性，这与基于古菌GDGTs（即TEX86）的温度代用指标的约束和验证有关。
Stratified lakes are important reservoirs of microbial diversity and provide habitats for niche differentiation of Archaea. In this study, we used a lipid biomarker/DNA-based approach to reveal the diversity and abundance of Archaea in the water column of Lake Challa (East Africa). Concentrations of intact polar lipid (IPL) crenarchaeol, a specific biomarker of Thaumarchaeota, were enhanced (1ng l(-1)) at the oxycline/nitrocline. The predominance of the more labile IPL hexose-phosphohexose crenarchaeol indicated the presence of an actively living community of Thaumarchaeota. Archaeal 16S rRNA clone libraries revealed the presence of thaumarchaeotal groups 1.1a and 1.1b at and above the oxycline. In the anoxic deep water, amoA gene abundance was an order of magnitude lower than at the oxycline and high abundance (approximate to 90ng l(-1)) of an IPL with the acyclic glycerol dialkyl glycerol tetraether (GDGT-0) was evident. The predominance of archaeal 16S rRNA sequences affiliated to the uncultured crenarchaeota groups 1.2 and miscellaneous crenarchaeotic group (MCG) points to an origin of GDGT-0 from uncultured crenarchaeota. This study demonstrates the importance of thermal stratification and nutrient availability in the distribution of archaeal groups in lakes, which is relevant to constrain and validate temperature proxies based on archaeal GDGTs (i.e. TEX86).