Urea and hypertonicity increase expression of heme oxygenase-1 in murine renal medullary cells.
Urea and hypertonicity increase expression of heme oxygenase-1 in murine renal medullary cells.
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尿素和高渗会增加小鼠肾髓质细胞中血红素加氧酶-1 的表达。
DOI：
10.1152/ajprenal.0358.2000
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Cohen,DM
中科院分区：
文献类型：
--
作者：
Tian,W;Bonkovsky,HL;Shibahara,S;Cohen,DM
First Published August 15, 2001; 10.1152/ajprenal. 00358.2001.—Epithelial cells derived from the mammalian kidney medulla are responsive to urea at the levels of signal transduction and gene regulation. Hybridization of RNA harvested from control- and urea-treated murine inner medullary collecting duct (mIMCD3) cells with a cDNA expression array encoding stress-responsive genes suggested that heme oxygenase (HO)-1 mRNA was upregulated by urea. RNase protection assay confirmed this upregulation; hypertonicity also increased HO-1 mRNA expression but neither hypertonic NaCl nor urea were effective in the nonrenal 3T3 cell line. The effect on HO-1 expression appeared to be transcriptionally mediated on the basis of mRNA half-life studies and reporter gene analyses using the promoters of both human and chicken HO-1. Although urea signaling resembles that of heavy metal signaling in other contexts, the effect of urea on HO-1 transcription was independent of the cadmium response element in this promoter. Urea-inducible HO-1 expression was sensitive to antioxidants but not to scavengers of nitric oxide. Urea also upregulated HO-1 protein expression and pharmacological inhibition of HO-1 action with zinc protoporphyrin-sensitized mIMCD3 cells to the adverse effects of hypertonicity but not to urea. Coupled with the prior observation of others that HO-1 expression increases along the renal corticomedullary gradient, these data suggest that HO-1 expression may comprise an element of the adaptive response to hypertonicity and/or urea in renal epithelial cells.
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DOI：
10.1152/ajprenal.2000.279.4.f728
发表时间：
2000-10
期刊：
American journal of physiology. Renal physiology
影响因子：
--
作者：
Mingyu Liang;A. J. Croatt;K. Nath
通讯作者：
Mingyu Liang;A. J. Croatt;K. Nath
DOI：
10.1152/ajprenal.1999.277.2.f176
发表时间：
1999-08
期刊：
American journal of physiology. Renal physiology
影响因子：
--
作者：
Xiao-Yan Yang;Zheng Zhang;D. Cohen
通讯作者：
Xiao-Yan Yang;Zheng Zhang;D. Cohen
鉴定负责镉介导的人血红素加氧酶基因诱导的顺式作用元件。
影响因子：
4.8
作者：
K. Takeda;S. Ishizawa;Masaaki Sato;T. Yoshida;Shigeki Shibahara
通讯作者：
Shigeki Shibahara
转染鸡胚肝细胞培养物中人血红素加氧酶 1 基因表达的调节。
DOI：
10.1016/s0167-4781(97)00028-6
发表时间：
1997
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Lu,TH;Pepe,JA;Gildemeister,OS;Tyrrell,RM;Bonkovsky,HL
通讯作者：
Bonkovsky,HL
金属介导的人血红素加氧酶-1 和金属硫蛋白 IIA 基因激活的差异控制。
影响因子：
3.1
作者：
K. Takeda;Hideaki Fujita;Shigeki Shibahara
通讯作者：
Shigeki Shibahara