Stress-Induced DNA Duplex Destabilization in Transcriptional Initiation
Stress-Induced DNA Duplex Destabilization in Transcriptional Initiation
复制标题
转录起始过程中应激诱导的 DNA 双链体不稳定
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
C. Benham
中科院分区：
文献类型：
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作者：
C. Benham
应激诱导的DNA双螺旋失稳(SIDD)参与了转录调控的几种机制。本文描述了一种根据DNA序列和施加的超螺旋应力来预测失稳位置和失稳程度的计算方法。这种方法被用来研究几个转录调控事件。这些包括IHF介导的大肠杆菌中基因表达的激活，人类c-myc基因转录启动的双峰控制，以及酵母中转录活性的最低要求的确定。与实验小组的合作确立了SIDD在每个进程中的核心作用。
Stress-induced destabilization of the DNA double helix (SIDD) is involved in several mechanisms by which transcription is regulated. This paper describes a computational method for predicting the locations and extents of destabilization as functions of DNA sequence and imposed superhelical stress. This method is used to investigate several transcriptional regulatory events. These include IHF-mediated activation of gene expression in E. coli, the bimodal control of the initiation of transcription from the human c-myc gene, and the determination of the minimal requirements for transcriptional activity in yeast. Collaborations with experimental groups have established the central role of SIDD in each of these processes.