Global analysis of mRNA localization reveals a prominent role in organizing cellular architecture and function
Global analysis of mRNA localization reveals a prominent role in organizing cellular architecture and function
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DOI：
10.1016/j.cell.2007.08.003
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发表时间：
2007-10-05
期刊：
影响因子：
64.5
通讯作者：
Krause, Henry M.
中科院分区：
文献类型：
--
作者：
Lecuyer, Eric;Yoshida, Hideki;Krause, Henry M.
尽管亚细胞mRNA运输已被证明是一种控制蛋白质分布的机制，但人们普遍认为大多数蛋白质定位发生在翻译之后。为了阐明这一点，我们开发并采用了一种高分辨率荧光原位杂交方法，以全面评估果蝇早期胚胎发生过程中mRNA定位的动态变化。令人惊讶的是，在分析的3370个基因中，71%的表达基因编码亚细胞定位的mRNA。观察到了数十种新的且显著的定位模式，这意味着存在同样多样的定位机制。mRNA分布与后续蛋白质定位及功能之间的紧密关联，表明mRNA定位在形成局部细胞机制中起主要作用。一个记录mRNA表达和定位动态的可搜索网络资源已经建立，它将成为剖析定位机制以及预测基因功能和相互作用的极有价值的工具。
Although subcellular mRNA trafficking has been demonstrated as a mechanism to control protein distribution, it is generally believed that most protein localization occurs subsequent to translation. To address this point, we developed and employed a high-resolution fluorescent in situ hybridization procedure to comprehensively evaluate mRNA localization dynamics during early Drosophila embryogenesis. Surprisingly, of the 3370 genes analyzed, 71% of those expressed encode subcellularly localized mRNAs. Dozens of new and striking localization patterns were observed, implying an equivalent variety of localization mechanisms. Tight correlations between mRNA distribution and subsequent protein localization and function, indicate major roles for mRNA localization in nucleating localized cellular machineries. A searchable web resource documenting mRNA expression and localization dynamics has been established and will serve as an invaluable tool for dissecting localization mechanisms and for predicting gene functions and interactions.