The effects of embryonic exposure to methanol on zebrafish growth, locomotor activity, and photoreceptor development
The effects of embryonic exposure to methanol on zebrafish growth, locomotor activity, and photoreceptor development
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发表时间：
2020
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通讯作者：
Jinling Fu;Ning Han;Yan Cheng;Guangjiang Shi
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作者：
Jinling Fu;Ning Han;Yan Cheng;Guangjiang Shi
Background: Methanol toxicity can lead to potentially fatal poisoning. We characterized how methanol affects zebrafish growth, external morphology, and locomotion, paying particular attention to photoreceptor development. Methods: Zebrafish embryos were divided into six groups and placed in hatching liquid for 6 hours. The embryos from five of the six groups were treated with varying concentrations (1%, 2%, 3%, 4%, and 5% by volume) of methanol from 6 to 24 hours post-fertilization. The toxic effects of methanol on zebrafish embryonic development were assessed based on the mortality rate, the hatching rate, and the morphological deformity rate. The effects of methanol on the locomotor activity of zebrafish embryo/larvae were assessed using autonomous motion and swimming behavior analyses. The effects of methanol on photoreceptor development were assessed using electron microscopy analyses. Results: Methanol increases mortality and induces growth retardation in zebrafish embryos. Zebrafish embryos exposed to 3% and 4% methanol during their early embryonic development displayed dramatic decreases in spontaneous tail movement at 24 hours post-fertilization and in the speed of their movement at 120 hours post-fertilization compared with the control group. Embryos exposed to a high (4%) concentration of methanol displayed morphological abnormalities in their photoreceptors. Conclusion: These findings suggest that methanol affects zebrafish growth and external morphology, and higher levels of methanol exposure can cause defects in locomotor activity and photoreceptor development.
