Production of proinflammatory cytokines by resident tissue macrophages after phagocytosis of apoptotic cells
Production of proinflammatory cytokines by resident tissue macrophages after phagocytosis of apoptotic cells
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DOI：
10.1006/cimm.2001.1824
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发表时间：
2001-07-10
影响因子：
4.3
通讯作者：
Kobayashi, Y
中科院分区：
文献类型：
--
作者：
Kurosaka, K;Watanabe, N;Kobayashi, Y
一般认为细胞凋亡与炎症无关。然而，我们发现PMA处理的THP-1细胞和人单核细胞衍生的巨噬细胞吞噬凋亡细胞导致促炎细胞因子的产生，特别是IL-8。这些巨噬细胞通过PMA处理或M-CSF处理获得，可能影响凋亡细胞吞噬后的细胞因子产生。为了排除这种可能性，我们在这项研究中使用了驻留组织巨噬细胞，如枯否细胞和肺泡巨噬细胞，并检查了吞噬凋亡细胞后细胞因子的产生。枯否细胞在mRNA水平产生促炎细胞因子MIP-2和TNF-α。用ELISA法检测MIP-2蛋白的表达。肺泡巨噬细胞吞噬凋亡细胞后也产生MIP-2蛋白。此外，凋亡的胸腺细胞诱导这些巨噬细胞的类似反应。这些发现确实支持了巨噬细胞在吞噬凋亡细胞后易于产生促炎细胞因子的观点。(C)北京：科学出版社.
It is generally believed that apoptosis is not associated with inflammation. However, we have found that phagocytosis of apoptotic cells by PMA-treated THP-1 cells and human monocyte-derived macrophages led to the production of proinflammatory cytokines, notably IL-8. These macrophages were obtained either by PMA treatment or by M-CSF treatment, possibly affecting the cytokine production after phagocytosis of apoptotic cells. In order to exclude the possibility, we employed resident tissue macrophages such as Kupffer cells and alveolar macrophages in this study and examined the production of cytokines after phagocytosis of apoptotic cells. Kupffer cells produced proinflammatory cytokines MIP-2 and TNF-alpha at the mRNA level. The MIP-2 protein was also detected by means of ELISA. Alveolar macrophages also produced the MIP-2 protein after phagocytosis of apoptotic cells. Furthermore, apoptotic thymocytes induced a similar response by these macrophages. These findings do support the notion that macrophages are apt to produce proinflammatory cytokines after phagocytosis of apoptotic cells. (C) 2001 Academic Press.