The role of interleukin-6 in the formation of the coronary vasculature.
The role of interleukin-6 in the formation of the coronary vasculature.
复制标题
DOI：
10.1017/s1431927609990353
复制
发表时间：
2009-10
期刊：
影响因子：
--
通讯作者：
Baudino TA
中科院分区：
文献类型：
--
作者：
Banerjee I;Fuseler JW;Souders CA;Bowers SL;Baudino TA
冠状动脉血管的形成和模式对心脏的发育和病理至关重要。细胞因子信号和生物力学负荷的改变可以改变心脏内血管的分布。血管的物理模式的变化可以对组织的压力-流量网络和关键生长和生存因子的分布关系产生重大影响。白细胞介素-6 （IL-6）是一种多效细胞因子，调节多种生物过程，包括血管生成。通过免疫组织学和心血管造影分析，我们验证了il -6缺失会导致血管密度降低以及血管分布改变的假设。此外，考虑到血管图案对压力流动和分布力学的影响，我们利用非欧几里得几何分形分析来量化il -6损失导致的图案变化。我们的分析显示，il -6的损失导致毛细血管密度降低和毛细血管间距离增加，但不会改变血管的大小或直径。我们还观察到，IL-6−/−冠状血管的分形维数（D值）显著增加，表明IL-6的损失改变了血管的模式。对冠状血管IL-6损失的表征可能有助于深入了解IL-6在血管床形成和模式中的作用。
The formation and the patterning of the coronary vasculature are critical to the development and pathology of the heart. Alterations in cytokine signaling and biomechanical load can alter the vascular distribution of the vessels within the heart. Changes in the physical patterning of the vasculature can have significant impacts on the relationships of the pressure-flow network and distribution of critical growth and survival factors to the tissue. Interleukin-6 (IL-6) is a pleiotropic cytokine that regulates several biological processes, including vasculogenesis. Using both immunohistological and cardioangiographic analyses, we tested the hypothesis that IL-6-loss will result in decreased vessel density, along with changes in vascular distribution. Moreover, given the impact of vascular patterning on pressure-flow and distribution mechanics, we utilized non-Euclidean geometrical fractal analysis to quantify the changes in patterning resulting from IL-6-loss. Our analyses revealed that IL-6-loss results in a decreased capillary density and increase in intercapillary distances, but does not alter vessel size or diameter. We also observed that the IL-6−/− coronary vasculature had a marked increase in fractal dimension (D value), indicating that IL-6-loss alters vascular patterning. Characterization of IL-6-loss on coronary vasculature may lend insight into the role of IL-6 in the formation and patterning of the vascular bed.