A detached-leaf bioassay for Xanthomonas campestris pv. pruni
A detached-leaf bioassay for Xanthomonas campestris pv. pruni
复制标题
野油菜黄单胞菌 pv 的离体叶生物测定。
DOI：
10.1094/phyto-75-1060
复制
发表时间：
1985
期刊：
影响因子：
3.2
通讯作者：
E. Civerolo
中科院分区：
文献类型：
--
作者：
P. Randhawa;E. Civerolo
用70%乙醇对桃苗的幼叶进行表面灭菌。用无针注射器将野油菜黄单胞菌(Xanthomonas campestris pv.pruni)的接种物(每毫升10“菌落单位)渗透到远轴侧的几个部位(约70CFU)。叶片在25℃的水琼脂中培养2wk，光照16小时。接种部位的叶片保持健康，并出现疾病症状。每个部位产生的病斑数量为
Young leaves of peach seedlings were detached and surface sterilized with 70% ethanol. Inoculum (10" colony-forming units [cfu] per milliliter) of Xanthomonas campestris pv. pruni was infiltrated with a needle-less syringe at several sites (approximately 70 cfu persite) on the abaxial side. The leaves were incubated on 0.5% water agar for 2 wk at 25 C under a 16-hr photoperiod. Leaves remained healthy and disease symptoms developed at the inoculation sites. The number of lesions produced at each site was