EFFECT OF MONOVALENT CATIONS ON OUABAIN INHIBITION OF SODIUM AND POTASSIUM ION ACTIVATED ADENOSINE TRIPHOSPHATASE
EFFECT OF MONOVALENT CATIONS ON OUABAIN INHIBITION OF SODIUM AND POTASSIUM ION ACTIVATED ADENOSINE TRIPHOSPHATASE
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DOI：
10.1021/bi00826a004
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发表时间：
1970-01-01
期刊：
影响因子：
2.9
通讯作者：
BARNETT, RE
中科院分区：
文献类型：
--
作者：
BARNETT, RE
罗纳德E.巴内特摘要：哇巴因对羊脑（Na++ K+）ATP酶的抑制作用在酶和哇巴因浓度下均为一级抑制。这意味着每个活性位点一个哇巴因的相互作用抑制酶。抑制的二级速率常数kn取决于钠离子和钾离子浓度。抑制率和结合率相同。...='class10'>对Na+和TCie（Na++ K+）ATP酶（ATP磷酸水解酶，EC 3.6. 1.3），被认为是大多数哺乳动物组织的碱性阳离子转运系统的组成部分，被强心苷如哇巴因特异性抑制（阿尔伯斯，1967）。关于酶-哇巴因复合物的稳定性存在一些争议。几份报告
Ronald E. Barnett abstract: Inhibition of the (Na++ K+) ATPase obtained from lamb brain by ouabain is first order in both enzyme and ouabain concentrations. This implies that the interaction of one ouabain per active site inhibits the enzyme. The second-order rate constant for inhibition, k „depends on both sodium and potassium ion concentrations. The rates of inhibition and binding are the same. The dependence of &¡ on sodium andTCie (Na++ K+) ATPase (ATP phosphohydrolase, EC 3.6. 1.3), which is believed to be an integral part of the alkali cation transport system of most mammalian tissues, is specifically inhibited bycardiac glycosides such as ouabain (Albers, 1967). There has been some controversy concerning the stability of the enzyme-ouabain complex. Several reports